Recombinant Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)
![Western blot - Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)](https://www.abcam.com/ps/products/255/ab255775/Images/ab255775-449270-AntiBOP1antibody6H11westernblotcaco2k562helahuman.jpg "Western blot - Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [6H11] to BOP1 - BSA and Azide free
- Suitable for: WB, ICC, IP
- Reacts with: Human
Overview
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Product name
Anti-BOP1 antibody [6H11] - BSA and Azide free
See all BOP1 primary antibodies -
Description
Rat monoclonal [6H11] to BOP1 - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: WB, ICC, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- WB: Caco-2, HeLa and K562 whole cell lysate. ICC; HeLa and K562 cells. IP: K562 whole cell lysate.
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General notes
ab255775 is the carrier-free version of ab252819. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab255775 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
6H11 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab255775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 84 kDa). | |
| ICC | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Target
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Function
Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. -
Sequence similarities
Belongs to the WD repeat BOP1/ERB1 family.
Contains 7 WD repeats. -
Cellular localization
Nucleus > nucleolus. Nucleus > nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 23246 Human
- Omim: 610596 Human
- SwissProt: Q14137 Human
- Unigene: 645279 Human
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Alternative names
- AU020183 antibody
- AW146150 antibody
- Block of proliferation 1 antibody
see all
Images
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Western blot - Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)All lanes : Anti-BOP1 antibody [6H11] (ab252819) at 1/5000 dilution
Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 84 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?This data was produced using the same clone but in a different formulation, ab252819.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 10 seconds; Lane 2: 59 seconds; Lane 3: 6 seconds.
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![Immunocytochemistry - Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)](https://www.abcam.com/ps/products/255/ab255775/Images/ab255775-449269-AntiBOP1antibody6H11immunocytochemistryhelahuman.jpg)
Immunocytochemistry - Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)This data was produced using the same clone but in a different formulation, ab252819.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling BOP1 with ab252819 at 1/50 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab252819 at a 1/50 dilution followed by ab150080 at a 1/1000 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.
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![Immunoprecipitation - Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)](https://www.abcam.com/ps/products/255/ab255775/Images/ab255775-449267-AntiBOP1antibody6H11immunoprecipitationk562human.jpg)
Immunoprecipitation - Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)This data was produced using the same clone but in a different formulation, ab252819.
BOP1 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab252819 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab252819 at 1/1,000 dilution. Goat Anti-Rat IgG (H+L), HRP (ab205720) was used at 1/10,000 dilution.
Lane 1: K562 whole cell lysate 10 µg.
Lane 2: ab252819 IP in K562 whole cell lysate.
Lane 3: Rat IgG1 (ab18407) instead of ab252819 in K562 whole cell lysate.
Blocking and dilution buffer and concentration/ 5% NFDM/TBST.
Exposure time: 3 seconds.
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![Immunocytochemistry - Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)](https://www.abcam.com/ps/products/255/ab255775/Images/ab255775-449268-AntiBOP1antibody6H11immunocytochemistryk562human.jpg)
Immunocytochemistry - Anti-BOP1 antibody [6H11] - BSA and Azide free (ab255775)This data was produced using the same clone but in a different formulation, ab252819.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labelling BOP1 with ab252819 at 1/50 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in K562 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab252819 at a 1/50 dilution followed by ab150080 at a 1/1000 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab255775 has been referenced in 1 publication.
- Hölzel M et al. Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation. J Cell Biol 170:367-78 (2005). PubMed: 16043514
