Key features and details
- Rabbit polyclonal to BOP1
- Suitable for: IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-BOP1 antibody
See all BOP1 primary antibodies
DescriptionRabbit polyclonal to BOP1
Tested applicationsSuitable for: IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Guinea pig, Cow, Pig, Rhesus monkey, Gorilla, Chinese hamster, Bat
Synthetic peptide corresponding to a region between residue 350 and 400 of Human BOP1 (NP_056016.1).
- HeLa whole cell lysate (ab150035).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
Sequence similaritiesBelongs to the WD repeat BOP1/ERB1 family.
Contains 7 WD repeats.
Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm.
- Information by UniProt
- AU020183 antibody
- AW146150 antibody
- Block of proliferation 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse teratoma tissues labelling BOP1 with ab86652 at 1/200 (1µg/ml). Detection: DAB.
Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86652 at 3 µg/mg lysate; Lane 2 represents control IgG IP. For the WB, ab86653 was used at 0.4 µg/ml.
Detection: Chemiluminescence with exposure time of 90 seconds.
All lanes : Anti-BOP1 antibody (ab86652) at 0.4 µg/ml
Lane 1 : immunoprecipitated BOP1
Lane 2 : control IgG
Observed band size: 101 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa, 55 kDa, 60-71 kDa. We are unsure as to the identity of these extra bands.
ab86652 has not yet been referenced specifically in any publications.