Overview

  • Product name

  • Description

    Rabbit polyclonal to BOP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 300 and 350 of Human BOP1 (NP_056016.1).

  • Positive control

    • HeLa and 293T whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
  • Sequence similarities

    Belongs to the WD repeat BOP1/ERB1 family.
    Contains 7 WD repeats.
  • Cellular localization

    Nucleus > nucleolus. Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AU020183 antibody
    • AW146150 antibody
    • Block of proliferation 1 antibody
    • Block of proliferation 1 protein antibody
    • Bop1 antibody
    • Bop1 block of proliferation 1 antibody
    • BOP1_HUMAN antibody
    • D18861 antibody
    • Erb1p antibody
    • Kiaa0124 antibody
    • MGC109114 antibody
    • mKIAA0124 antibody
    • Ribosome biogenesis protein BOP1 antibody
    see all

Images

  • All lanes : Anti-BOP1 antibody (ab86653) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 84 kDa
    Observed band size: 100,110 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 120 kDa, 65 kDa, 90 kDa. We are unsure as to the identity of these extra bands.



    Detection: Chemiluminescence with exposure time of 90 seconds
  • Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86653 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with exposure time of 90 seconds

References

ab86653 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab86653.
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