Anti-Bordetella pertussis toxin subunit S2 antibody [20.6] (ab27158)

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Key features and details

  • Mouse monoclonal [20.6] to Bordetella pertussis toxin subunit S2
  • Suitable for: ELISA, WB
  • Reacts with: Bordetella pertussis
  • Isotype: IgG1

Overview

  • Product name

    Anti-Bordetella pertussis toxin subunit S2 antibody [20.6]

  • Description

    Mouse monoclonal [20.6] to Bordetella pertussis toxin subunit S2

  • Host species

    Mouse

  • Specificity

    Reacts with the intact toxin as well as the S2 subunit. Some reactivity towards the toxoid is also present.

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Species reactivity

    Reacts with: Bordetella pertussis

  • Immunogen

    Pertussis Toxin.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.40
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    20.6

  • Myeloma

    x63-Ag8.653

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab27158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/100000.
WB
1/1000.
Notes
ELISA
1/100000.
WB
1/1000.

Target

  • Relevance

    Pertussis toxin (islet activating protein) is the major protein toxin produced by virulent strains of Bordetella pertussis. As revealed by polyacrylamide gel electrophoresis, the purified protein consists of five dissimilar subunits: S1 (MW 28,000), S2 (MW 23,000), S3 (MW 22,000), S4 (MW 11,700) and S5 (MW 9,300), in a molar ratio of 1:1:1:2:1. The A protomer, S1 is responsible for the enzymatic activity of the toxin. Together, S2, S3, S4 and S5 comprise the B oligomer, responsible for binding the toxin to the cell surface.

  • Database links

    • Alternative names

      • B.pertussis S2 antibody
      • B.pertussis toxin subunit S2 antibody
      • Bordetella pertussis S2 antibody
      • IAP S2 antibody
      • Islet activating protein S2 antibody
      • Pertussis toxin S2 antibody
      • Pertussis toxin subunit 2 antibody
      • Ptx B antibody
      • PTX S2 antibody
      • PtxB antibody
      see all

    References (1)

    Publishing research using ab27158? Please let us know so that we can cite the reference in this datasheet.

    ab27158 has been referenced in 1 publication.

    • Ibsen PH The effect of formaldehyde, hydrogen peroxide and genetic detoxification of pertussis toxin on epitope recognition by murine monoclonal antibodies. Vaccine 14:359-68 (1996). PubMed: 8735545

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