Anti-Bordetella pertussis toxin subunit S2 antibody [20.6] (ab27158)
Key features and details
- Mouse monoclonal [20.6] to Bordetella pertussis toxin subunit S2
- Suitable for: ELISA, WB
- Reacts with: Bordetella pertussis
- Isotype: IgG1
Overview
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Product name
Anti-Bordetella pertussis toxin subunit S2 antibody [20.6] -
Description
Mouse monoclonal [20.6] to Bordetella pertussis toxin subunit S2 -
Host species
Mouse -
Specificity
Reacts with the intact toxin as well as the S2 subunit. Some reactivity towards the toxoid is also present. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Bordetella pertussis -
Immunogen
Pertussis Toxin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
20.6 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
1/100000.
|
|
| WB |
1/1000.
|
| Notes |
|---|
|
ELISA
1/100000. |
|
WB
1/1000. |
Target
-
Relevance
Pertussis toxin (islet activating protein) is the major protein toxin produced by virulent strains of Bordetella pertussis. As revealed by polyacrylamide gel electrophoresis, the purified protein consists of five dissimilar subunits: S1 (MW 28,000), S2 (MW 23,000), S3 (MW 22,000), S4 (MW 11,700) and S5 (MW 9,300), in a molar ratio of 1:1:1:2:1. The A protomer, S1 is responsible for the enzymatic activity of the toxin. Together, S2, S3, S4 and S5 comprise the B oligomer, responsible for binding the toxin to the cell surface. -
Database links
- Entrez Gene: 2665069 Bordetella pertussis
- SwissProt: P04978 Bordetella pertussis
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Alternative names
- B.pertussis S2 antibody
- B.pertussis toxin subunit S2 antibody
- Bordetella pertussis S2 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab27158 has been referenced in 1 publication.
- Ibsen PH The effect of formaldehyde, hydrogen peroxide and genetic detoxification of pertussis toxin on epitope recognition by murine monoclonal antibodies. Vaccine 14:359-68 (1996). PubMed: 8735545