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  1. Link

    bordetella-pertussis-toxinptxd-antibody-501c2-ab37684.pdf

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Microbiology Organism Bacteria Gram negative Bacteria Other gram-ve bacteria
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Anti-Bordetella pertussis toxin/ptxD antibody [50.1C2] (ab37684)

  • Datasheet
Submit a review Submit a question References (1)

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  • Datasheet
  • References (1)
  • Protocols

Overview

  • Product name

    Anti-Bordetella pertussis toxin/ptxD antibody [50.1C2]
    See all Bordetella pertussis toxin/ptxD primary antibodies
  • Description

    Mouse monoclonal [50.1C2] to Bordetella pertussis toxin/ptxD
  • Host species

    Mouse
  • Specificity

    Ab37684 reacts with different epitope than ab37574, ab37686 and ab37687.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Bordetella pertussis
  • Immunogen

    Pertussis Toxin

  • General notes

     This product was previously labelled as Bordetella pertussis toxin

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Constituents: 2.9% Sodium chloride, 0.0268% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    50.1C2
  • Myeloma

    x63-Ag8.653
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Microbiology
    • Organism
    • Bacteria
    • Gram negative Bacteria
    • Other gram-ve bacteria

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

Our Abpromise guarantee covers the use of ab37684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 9.3 kDa.

Target

  • Relevance

    Pertussis toxin contains five different chains, S1-S5. They are organized into 2 functional subunits: A, composed of S1 (which is toxic) and B, containing S2, S3, S5, and two copies of S4 (B binds to the membrane receptors). Dimers of S2-S4 and S3-S4 are held together by S5
  • Alternative names

    • IAP S1 antibody
    • Islet-activating protein S1 antibody
    • NAD-dependent ADP-ribosyltransferase antibody

Protocols

  • Western blot protocols

Datasheets and documents

    • Datasheet
  • References

    This product has been referenced in:

    • Ibsen PH The effect of formaldehyde, hydrogen peroxide and genetic detoxification of pertussis toxin on epitope recognition by murine monoclonal antibodies. Vaccine 14:359-68 (1996). Read more (PubMed: 8735545) »
    See 1 Publication for this product

    Publishing research using ab37684? Please let us know so that we can cite the reference in this datasheet.

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