Key features and details
- Mouse polyclonal to Borealin/CDCA8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Borealin/CDCA8 antibody
See all Borealin/CDCA8 primary antibodies
DescriptionMouse polyclonal to Borealin/CDCA8
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length native Borealin/CDCA8 (purified) (Human)
- Borealin/CDCA8 transfected 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab67126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).|
FunctionComponent of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
Sequence similaritiesBelongs to the borealin family.
Developmental stageCell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.
DomainThe C-terminal region (aa 207-280) represents the dimerization motif.
modificationsPhosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
Cellular localizationNucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
- Information by UniProt
- BOR antibody
- BOREA_HUMAN antibody
- Borealin antibody
All lanes : Anti-Borealin/CDCA8 antibody (ab67126) at 1/500 dilution
Lane 1 : Borealin/CDCA8 transfected 293T cell lysate
Lane 2 : Untransfected 293T cell lysate
All lanes : Goat anti mouse IgG (H&L)-HRP conjugate secondary
antibody at 1/2500 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
ab67126 has been referenced in 1 publication.
- Pike T et al. PKC? switches Aurora B specificity to exit the abscission checkpoint. Nat Commun 7:13853 (2016). WB, ICC/IF . PubMed: 28004745