Anti-Borealin/CDCA8 antibody (ab74473)
Key features and details
- Rabbit polyclonal to Borealin/CDCA8
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Borealin/CDCA8 antibody
See all Borealin/CDCA8 primary antibodies -
Description
Rabbit polyclonal to Borealin/CDCA8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Rat kidney tissue lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa). |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. -
Sequence similarities
Belongs to the borealin family. -
Developmental stage
Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase. -
Domain
The C-terminal region (aa 207-280) represents the dimerization motif. -
Post-translational
modificationsPhosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells. - Information by UniProt
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Database links
- Entrez Gene: 500545 Rat
- SwissProt: Q6AXW0 Rat
- Unigene: 14970 Rat
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Alternative names
- BOR antibody
- BOREA_HUMAN antibody
- Borealin antibody
see all
Images
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Lane 1 : Anti-Borealin/CDCA8 antibody (ab74473) at 1 µg/ml
Lane 2 : Anti-Borealin/CDCA8 antibody (ab74473) at 2 µg/ml
All lanes : Rat kidney lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 31 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted? -
Immunofluorescence of CDCA8 in Rat Kidney cells using ab74473 at 20 ug/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab74473 has been referenced in 1 publication.
- Kim HJ et al. Cyclin-dependent kinase 1 activity coordinates the chromatin associated state of Oct4 during cell cycle in embryonic stem cells. Nucleic Acids Res 46:6544-6560 (2018). PubMed: 29901724