Key features and details
- Rabbit polyclonal to Borealin/CDCA8
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Borealin/CDCA8 antibody
See all Borealin/CDCA8 primary antibodies
DescriptionRabbit polyclonal to Borealin/CDCA8
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide of 19 amino acids taken from near the N-terminus of Human Borealin/CDCA8. (NP_060571)
- Rat kidney tissue lysate
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
FunctionComponent of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
Sequence similaritiesBelongs to the borealin family.
Developmental stageCell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.
DomainThe C-terminal region (aa 207-280) represents the dimerization motif.
modificationsPhosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
Cellular localizationNucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
- Information by UniProt
- BOR antibody
- BOREA_HUMAN antibody
- Borealin antibody
Lane 1 : Anti-Borealin/CDCA8 antibody (ab74473) at 1 µg/ml
Lane 2 : Anti-Borealin/CDCA8 antibody (ab74473) at 2 µg/ml
All lanes : Rat kidney lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 31 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Immunofluorescence of CDCA8 in Rat Kidney cells using ab74473 at 20 ug/ml.
ab74473 has been referenced in 1 publication.
- Kim HJ et al. Cyclin-dependent kinase 1 activity coordinates the chromatin associated state of Oct4 during cell cycle in embryonic stem cells. Nucleic Acids Res 46:6544-6560 (2018). PubMed: 29901724