Overview

  • Product name

    Anti-Borealin/CDCA8 antibody
    See all Borealin/CDCA8 primary antibodies
  • Description

    Rabbit polyclonal to Borealin/CDCA8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide of 19 amino acids taken from near the N-terminus of Human Borealin/CDCA8. (NP_060571)

  • Positive control

    • Rat kidney tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab74473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
  • Sequence similarities

    Belongs to the borealin family.
  • Developmental stage

    Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.
  • Domain

    The C-terminal region (aa 207-280) represents the dimerization motif.
  • Post-translational
    modifications

    Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
    Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • BOR antibody
    • BOREA_HUMAN antibody
    • Borealin antibody
    • CDCA8 antibody
    • Cell division cycle associated 8 antibody
    • cell division cycle associated protein 8 antibody
    • Cell division cycle-associated protein 8 antibody
    • Dasra B antibody
    • Dasra-B antibody
    • DasraB antibody
    • FLJ10468 antibody
    • FLJ12042 antibody
    • hDasra B antibody
    • hDasra-B antibody
    • MESRGP antibody
    • OTTHUMP00000004514 antibody
    • pluripotent embryonic stem cell related gene 3 protein antibody
    • Pluripotent embryonic stem cell-related gene 3 protein antibody
    see all

Images

  • Lane 1 : Anti-Borealin/CDCA8 antibody (ab74473) at 1 µg/ml
    Lane 2 : Anti-Borealin/CDCA8 antibody (ab74473) at 2 µg/ml

    All lanes : Rat kidney lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 31 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence of CDCA8 in Rat Kidney cells using ab74473 at 20 ug/ml.

References

This product has been referenced in:

  • Kim HJ  et al. Cyclin-dependent kinase 1 activity coordinates the chromatin associated state of Oct4 during cell cycle in embryonic stem cells. Nucleic Acids Res 46:6544-6560 (2018). Read more (PubMed: 29901724) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you very much for your call today and for letting us know about the trouble with these antibodies.

I am very sorry that these antibodies did not work as stated on the datasheet. As we discussed, I'm sending ab70910 free of charge on the order ***which should arrive on Wednesday, and also ab134112 on the order *** which we expect to arrive on Friday.

Please keep me updated about the results with these replacement antibodies, and let me know if there is anything else that we can do for you.

Read More
Application
Western blot
Sample
Human Purified protein (GST-Borealin of human origin)
Loading amount
1 µg
Specification
GST-Borealin of human origin
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

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Verified customer

Submitted Apr 07 2009

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