Overview

  • Product name

    Anti-Borrelia burgdorferi antibody (FITC)
    See all Borrelia burgdorferi primary antibodies
  • Description

    Rabbit polyclonal to Borrelia burgdorferi (FITC)
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    This antibody antibody reacts with Borrelia burgdorferi in bacterial and infected tissue samples.
  • Tested applications

    Suitable for: IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Borrelia burgdorferi whole cell preparation.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab69252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/10.
ICC/IF 1/10.

Target

  • Relevance

    Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response. B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.
  • Alternative names

    • B burgdorferi antibody
    • B. burgdorferi antibody
    • B.burgdorferi antibody
    • Lyme disease antibody

References

ab69252 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.Ab69252 is batch tested using direct immunofluorescence in a microscopy assay (ICC/IF). This is a semi-quantitative assay, but we estimate approximately 20% variability between lots. This variability is due to the fact that ab69252 is a polyclonal antibody raised against a whole cell preparation. For a less batch variable product, I would recommend using one of our mousemonoclonal antibodies (ab23571 or ab128165)with our EasyLink FITC conjugation kit ab102884.

Our EasyLink conjugation kits allow for rapid and efficient conjugation of antibodies to a number of fluorophores. The antibody ab23571 is supplied at a low concentration, so it would need to be first prepared with the concentration kit ab102776. For more information, please see https://www.abcam.com/EasyLink

I hope this helps, please let me know if you need any additional information or assistance.

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Answer

Yes, both ab69252 (FITC labeled) and ab69158 (Biotin labeled) use the same antibody.

As for ab69158, the molar ratio is not determined but based on the procdure use, it should be about 7-8 moles of biotin/mole of IgG.
Please feel free to contact us with any additional questions or comments.

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