Reacts with Treponema pallidum, B. hermsii and B. parkerii.
Not yet tested in other species.
Whole cell preparation from B. burgdorferi.
Purified IgG fraction of antiserum covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.
Shipped at 4°C. Store at +4°C.
Preservative: 0.002% Thimerosal (merthiolate)
Constituents: PBS, 10mg/ml BSA
Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/200 - 1/1000.
IHC-P: 1/20 - 1/200.
WB: 1/20 - 1/200. Detects bands of approximately 83 kDa, 41 kDa, 34 kDa, 31 kDa and additional low MW bands. Dilution optimised using Chromogenic detection.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response.
B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.