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Immunology Immunoglobulins Heavy Chain IgG
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Anti-Borrelia burgdorferi IgG ELISA Kit (ab178635)

  • Datasheet
  • SDS
  • Protocol Booklet
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Serologic ELISA assay principle

    Key features and details

    • Sensitivity: 98.6 %
    • Sample type: Cit plasma, Hep Plasma, Other biological fluids, Serum
    • Detection method: Colorimetric
    • Assay type: Indirect

    Overview

    • Product name

      Anti-Borrelia burgdorferi IgG ELISA Kit
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Pos serum 7 4.6%
      Inter-assay
      Sample n Mean SD CV%
      Pos serum 3 4%
    • Sample type

      Serum, Other biological fluids, Hep Plasma, Cit plasma
    • Assay type

      Indirect
    • Sensitivity

      98.6 %
    • Assay duration

      Multiple steps standard assay
    • Product overview

      Abcam’s anti- Borrelia burgdorferi IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Borrelia burgdorferi in Human serum, plasma and CSF.

       

      A 96-well plate has been precoated with Borrelia burgdorferi antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Borrelia burgdorferi antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Borrelia burgdorferi IgG sample captured in plate.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      20X Washing Solution White cap 1 x 50ml
      Borrelia burgdorferi (IgG) Coated Microplate (12 x 8 wells) 1 unit
      Borrelia burgdorferi Anti-IgG HRP Conjugate 1 x 20ml
      Borrelia burgdorferi Cut-off Control 1 x 3ml
      Borrelia burgdorferi Negative Control 1 x 2ml
      Borrelia burgdorferi Positive Control 1 x 2ml
      Cover Foil 1 unit
      IgG Sample Diluent 1 x 100ml
      Stop Solution red cap 1 x 15ml
      Strip holder 1 unit
      TMB Substrate Solution Yellow cap 1 x 15ml
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Microbiology
      • Organism
      • Bacteria
      • Gram negative Bacteria
      • Borrelia
      • Kits/ Lysates/ Other
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      • ELISA Kits
      • ELISA Kits
      • Pathogen specific immunoglobulins ELISA kits
      • Kits/ Lysates/ Other
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      • Infectious disease ELISA kits
    • Relevance

      Borrelia burgdorferi is a bacterial species of the spirochete class of the genus Borrelia, which has a diderm (double-membrane) envelope. B. burgdorferi is predominant in North America, but also exists in Europe, and is the agent of Lyme disease. Borrelia burgdorferi is gram negative only by default because safranin is the last dye used, but in reality spirochetes are not classified as either gram positive or negative.

    Images

    • Serologic ELISA assay principle
      Serologic ELISA assay principle
      Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab178635? Please let us know so that we can cite the reference in this datasheet.

    ab178635 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    I have a question regarding the Abcam anti-borrelia burgdorferi IgG ELISA kit, catalogue number ab178635
    Are the wells of the microtitre plate coated with native antigen or recombinant antigen?
    Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Oct 23 2014

    Answer

    Thank you for contacting us.

    The microtitre plate wells were coated with recombinant antigen.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Padamjeet Singh

    Abcam Scientific Support

    Answered on Oct 23 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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