Key features and details
- Mouse monoclonal [230F9] to BOTOX
- Suitable for: ICC/IF, Inhibition Assay
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-BOTOX antibody [230F9]
See all BOTOX primary antibodies
DescriptionMouse monoclonal [230F9] to BOTOX
SpecificityBoTox-A (1280-1292) 100%; BoTox-A 100% Does not cross react with BoTox-B, BoTox-C, BoTox-E or BoTox-F (1272-1280)
Tested applicationsSuitable for: ICC/IF, Inhibition Assaymore details
Species reactivityReacts with: Species independent
Synthetic peptide corresponding to BOTOX (C terminal).
Previously labelled as Clostridium botulinum A Toxoid.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Purification notesSterile filtered culture supernatant.
Our Abpromise guarantee covers the use of ab40786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|Inhibition Assay||Use at an assay dependent dilution. Inhibits BoTox-A binding to Neuro2A cells treated with GT1b ganglioside which can be eliminated by prior incubation with synthetic peptide immunogen.|
RelevanceClostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.
- Atx antibody
- BotA antibody
- BOTOX antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab40786 has been referenced in 1 publication.
- Bräuer L et al. Staphylococcus aureus and Pseudomonas aeruginosa express and secrete human surfactant proteins. PLoS One 8:e53705 (2013). PubMed: 23349731