Anti-BOTOX antibody [230F9] (ab40786)
Key features and details
- Mouse monoclonal [230F9] to BOTOX
- Suitable for: ICC/IF, Inhibition Assay
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-BOTOX antibody [230F9]
See all BOTOX primary antibodies -
Description
Mouse monoclonal [230F9] to BOTOX -
Host species
Mouse -
Specificity
BoTox-A (1280-1292) 100%; BoTox-A 100% Does not cross react with BoTox-B, BoTox-C, BoTox-E or BoTox-F (1272-1280) -
Tested applications
Suitable for: ICC/IF, Inhibition Assaymore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to BOTOX (C terminal).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Sterile filtered culture supernatant. -
Clonality
Monoclonal -
Clone number
230F9 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent dilution.
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| Inhibition Assay |
Use at an assay dependent dilution. Inhibits BoTox-A binding to Neuro2A cells treated with GT1b ganglioside which can be eliminated by prior incubation with synthetic peptide immunogen.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent dilution. |
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Inhibition Assay
Use at an assay dependent dilution. Inhibits BoTox-A binding to Neuro2A cells treated with GT1b ganglioside which can be eliminated by prior incubation with synthetic peptide immunogen. |
Target
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Relevance
Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25. -
Alternative names
- Atx antibody
- BotA antibody
- BOTOX antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab40786 has been referenced in 1 publication.
- Bräuer L et al. Staphylococcus aureus and Pseudomonas aeruginosa express and secrete human surfactant proteins. PLoS One 8:e53705 (2013). PubMed: 23349731
