Anti-BOTOX antibody [230F9] (ab40786)

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Key features and details

  • Mouse monoclonal [230F9] to BOTOX
  • Suitable for: ICC/IF, Inhibition Assay
  • Reacts with: Species independent
  • Isotype: IgG

Overview

  • Product name

    Anti-BOTOX antibody [230F9]
    See all BOTOX primary antibodies

  • Description

    Mouse monoclonal [230F9] to BOTOX

  • Host species

    Mouse

  • Specificity

    BoTox-A (1280-1292) 100%; BoTox-A 100% Does not cross react with BoTox-B, BoTox-C, BoTox-E or BoTox-F (1272-1280)

  • Tested applications

    Suitable for: ICC/IF, Inhibition Assaymore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide corresponding to BOTOX (C terminal).

  • General notes

    Previously labelled as Clostridium botulinum A Toxoid.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Constituent: Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Purification notes

    Sterile filtered culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    230F9

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
Inhibition Assay Use at an assay dependent dilution. Inhibits BoTox-A binding to Neuro2A cells treated with GT1b ganglioside which can be eliminated by prior incubation with synthetic peptide immunogen.

Target

  • Relevance

    Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.

  • Alternative names

    • Atx antibody
    • BotA antibody
    • BOTOX antibody
    • Botulinum neurotoxin type A antibody
    • Botulism A toxoid antibody
    • Clostridium botulinum A Toxoid antibody
    see all

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab40786? Please let us know so that we can cite the reference in this datasheet.

ab40786 has been referenced in 1 publication.

  • Bräuer L  et al. Staphylococcus aureus and Pseudomonas aeruginosa express and secrete human surfactant proteins. PLoS One 8:e53705 (2013). PubMed: 23349731

Customer reviews and Q&As

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Question

this is to have some clarification about the product Anti-Clostridium botulinum A Toxoid antibody [230F9] (ab40786).
Does it reacts well with the whole toxin? We need for an antibody that can recognize the Botulinum toxin inside the cells to look whether cells can internalize the toxin. Moreover it is tested for IF/ICC: do you think it could work also in western blot?

Thanks for your assistance

Best regards,

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Answer

Thank you for contacting us.

The immunogen of antibody ab40786 is a "synthetic peptide analogue of the carboxyl terminal region of BoTox-A", http://www.uniprot.org/uniprot/P10845.

We have not tested this antibody in WB, if you are interested in testing this ba in WB then you might be interested in our Abtrial offer. Please click the link below for more information.

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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