Anti-BOTOX antibody (ab20436)

Submit a review Q&A (3)

Key features and details

  • Rabbit polyclonal to BOTOX
  • Suitable for: Dot blot, ELISA, WB
  • Reacts with: Species independent
  • Isotype: IgG

Overview

  • Product name

    Anti-BOTOX antibody
    See all BOTOX primary antibodies

  • Description

    Rabbit polyclonal to BOTOX

  • Host species

    Rabbit

  • Specificity

    Clostridium botulinum Type A Hemagglutinin 33

  • Tested applications

    Suitable for: Dot blot, ELISA, WBmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Full length native protein (purified) corresponding to BOTOX.

  • General notes

    Previously labelled as Clostridium botulinum A Toxoid.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Constituents: 2.68% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution. Predicted molecular weight: 149 kDa.
Notes
Dot blot
Use at an assay dependent dilution.
ELISA
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution. Predicted molecular weight: 149 kDa.

Target

  • Relevance

    Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.

  • Alternative names

    • Atx antibody
    • BotA antibody
    • BOTOX antibody
    • Botulinum neurotoxin type A antibody
    • Botulism A toxoid antibody
    • Clostridium botulinum A Toxoid antibody
    see all

References (0)

Publishing research using ab20436? Please let us know so that we can cite the reference in this datasheet.

ab20436 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

I want to find the sequence of the DNA that was used to make the antibody to determine whether I will be able to use a/any antibody.

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Answer


The antibody was raised against formaldehyde inactivated Type A Hemagglutinin 33, thus I am not certain if the sequence has been determined for the immunogen. The epitope has definitely not been mapped.

I will check with the lab if any additional information is available and let you know.

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Question

Was the immunogen purified before it was injected into the host rabbit?

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Answer

Thank you for your inquiry and I am so sorry that this took so long to get back to you. I just heard back from the supplying laboratory that yes, the immunogen for ab20436, Rabbit anti C. botulinum Type A Hemagglutinin-33, was purifed prior to injecting the rabbit. I hope this information helps. Please contact us with any other questions.

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Question

I have a few questions regarding the products ab20436 & ab20641. Firstly, what is the difference between these two antibodies? I see that one of them says specificity for Hemagglutinin, but don't realize the difference. I would like to use this to do IHC on BOTOX TYPE A. Is it possible to use one of these antibodies for this? Also, could you kindly email me the detection limits (either in ng/ml or U/ml). Thank you so much for your help. I would really appreciate a prompt response.

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Answer

Thank you for your enquiry and for your interest in our products. Clostridium botulinum neurotoxin is produced along with a group of neurotoxin-associated proteins which protect the neurotoxin from low pH and proteases of the gastrointestinal tract. The neurotoxin together with the neurotoxin-associated proteins make up the neurotoxin complex. Hemagglutinin-33 is one of the neurotoxin-associated proteins. Ab20436 is specific for C. botulinum Hemagglutinin-33. Ab20641 is specific for the C. botulinum Neurotoxin. These antibodies have not been tested in IHC. I do not have any information about BOTOX cosmetic and the detection limits have not been determined using these antibodies.

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