Anti-BOTOX antibody (ab20641)

Reviews (1)Q&A (3)References (4)

Key features and details

  • Rabbit polyclonal to BOTOX
  • Suitable for: ELISA, WB
  • Reacts with: Species independent
  • Isotype: IgG

Overview

  • Product name

    Anti-BOTOX antibody
    See all BOTOX primary antibodies

  • Description

    Rabbit polyclonal to BOTOX

  • Host species

    Rabbit

  • Specificity

    Clostridium botulinum Type A Neurotoxin.

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Full length protein corresponding to BOTOX.

  • General notes

    Previously labelled as Clostridium botulinum A Toxoid.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Constituents: 2.68% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/10000.
WB (1)
Use at an assay dependent dilution. Predicted molecular weight: 149 kDa.
Notes
ELISA
1/10000.
WB
Use at an assay dependent dilution. Predicted molecular weight: 149 kDa.

Target

  • Relevance

    Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.

  • Alternative names

    • Atx antibody
    • BotA antibody
    • BOTOX antibody
    • Botulinum neurotoxin type A antibody
    • Botulism A toxoid antibody
    • Clostridium botulinum A Toxoid antibody
    see all

References (4)

Publishing research using ab20641? Please let us know so that we can cite the reference in this datasheet.

ab20641 has been referenced in 4 publications.

  • Bagramyan K  et al. Substrates and controls for the quantitative detection of active botulinum neurotoxin in protease-containing samples. Anal Chem 85:5569-76 (2013). PubMed: 23656526
  • Scotcher MC  et al. Epitope characterization and variable region sequence of f1-40, a high-affinity monoclonal antibody to botulinum neurotoxin type a (Hall strain). PLoS ONE 4:e4924 (2009). WB . PubMed: 19290051
  • Scotcher MC  et al. Characterization of the epitope region of F1-2 and F1-5, two monoclonal antibodies to Botulinum neurotoxin type A. Hybridoma (Larchmt) 28:315-25 (2009). WB . PubMed: 19857112
  • Bagramyan K  et al. Attomolar detection of botulinum toxin type A in complex biological matrices. PLoS ONE 3:e2041 (2008). PubMed: 18446228

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

What type of ELISA was done to validate these antibodies? How much protein was used and can we provide protocol details?

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Answer

For catalog # ab20641 and ab20786 a direct ELISA was performed. The limit of detection (LOD) with direct ELISA is greater than 1:10,000 on both products with no amplification using OPD (o-phenylenediamine dihydrochloride) development and HRP conjugated 2nd antibody.
This is the most detail that we have available, and we recommend optimization of the reagents/antibodies in your specific application.

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Western blot abreview for Anti-BOTOX antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Clostridium botulinum Purified protein (cell lysate)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
5 µg
Specification
cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Dr. chung sei kim

Verified customer

Submitted Jun 20 2012

Question

No signal in ELISA with ab20641. No signal in WB with ab7202.

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Answer

Thank you for your phone call today, and I apologize for any miscommunication with my colleague yesterday. Your credit note ID for ab20641 is: ***. Your credit note ID for ab7202 is: ***. I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original orders and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

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Question

We previously ordered ab20641-250, and have product information describing this antibody as anti-"toxin." However, your website now calls this same product anti-"toxoid," has the product changed recently, or only the information describing the product? We are planning to repeat an experiment, and would like to be certain that we are using the same reagent. Any information you have regarding this situation would be much appreciated.

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Answer

Thank you for your enquiry. I can confirm that the immunogen for this antibody (ab20641) has not changed. A toxoid is an attenuated/inactivated toxin. The Clostridium botulinum Type A Neurotoxin is inactivated using formaldehyde before it is used as an immunogen to create this antibody. Fairly recently, a decision was taken to change toxin to toxoid on the datasheet as this was considered to be a more accurate description. I hope this information is helpful. Please do not hesitate to contact us again should you have any further questions.

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