Anti-BOTOX antibody (ab20641)

Reviews (1)Q&A (3)References (4)

Overview

  • Product name
    Anti-BOTOX antibody
    See all BOTOX primary antibodies
  • Description
    Rabbit polyclonal to BOTOX
  • Host species
    Rabbit
  • Specificity
    Clostridium botulinum Type A Neurotoxin.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Immunogen

    Full length protein corresponding to BOTOX.

  • General notes

    Previously labelled as Clostridium botulinum A Toxoid.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Constituents: 2.68% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB Use at an assay dependent dilution. Predicted molecular weight: 149 kDa.

Target

  • Relevance
    Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.
  • Alternative names
    • Atx antibody
    • BotA antibody
    • BOTOX antibody
    • Botulinum neurotoxin type A antibody
    • Botulism A toxoid antibody
    • Clostridium botulinum A Toxoid antibody
    see all

References

This product has been referenced in:
  • Bagramyan K  et al. Substrates and controls for the quantitative detection of active botulinum neurotoxin in protease-containing samples. Anal Chem 85:5569-76 (2013). Read more (PubMed: 23656526) »
  • Scotcher MC  et al. Epitope characterization and variable region sequence of f1-40, a high-affinity monoclonal antibody to botulinum neurotoxin type a (Hall strain). PLoS ONE 4:e4924 (2009). WB . Read more (PubMed: 19290051) »
See all 4 Publications for this product

Publishing research using ab20641? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

What type of ELISA was done to validate these antibodies? How much protein was used and can we provide protocol details?

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Answer

For catalog # ab20641 and ab20786 a direct ELISA was performed. The limit of detection (LOD) with direct ELISA is greater than 1:10,000 on both products with no amplification using OPD (o-phenylenediamine dihydrochloride) development and HRP conjugated 2nd antibody.
This is the most detail that we have available, and we recommend optimization of the reagents/antibodies in your specific application.

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Western blot abreview for Anti-BOTOX antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Western blot
Sample
Clostridium botulinum Purified protein (cell lysate)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
5 µg
Specification
cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Dr. chung sei kim

Verified customer

Submitted Jun 20 2012

Question

No signal in ELISA with ab20641. No signal in WB with ab7202.

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Answer

Thank you for your phone call today, and I apologize for any miscommunication with my colleague yesterday. Your credit note ID for ab20641 is: ***. Your credit note ID for ab7202 is: ***. I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original orders and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

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Question

We previously ordered ab20641-250, and have product information describing this antibody as anti-"toxin." However, your website now calls this same product anti-"toxoid," has the product changed recently, or only the information describing the product? We are planning to repeat an experiment, and would like to be certain that we are using the same reagent. Any information you have regarding this situation would be much appreciated.

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Answer

Thank you for your enquiry. I can confirm that the immunogen for this antibody (ab20641) has not changed. A toxoid is an attenuated/inactivated toxin. The Clostridium botulinum Type A Neurotoxin is inactivated using formaldehyde before it is used as an immunogen to create this antibody. Fairly recently, a decision was taken to change toxin to toxoid on the datasheet as this was considered to be a more accurate description. I hope this information is helpful. Please do not hesitate to contact us again should you have any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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