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    botox-antibody-ab20641.pdf

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Microbiology Toxin Bacterial Toxin Clostridium Toxin
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Anti-BOTOX antibody (ab20641)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (4)

Product price, shipping and contact information

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Abpromise

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Key features and details

  • Rabbit polyclonal to BOTOX
  • Suitable for: ELISA, WB
  • Reacts with: Species independent
  • Isotype: IgG

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Overview

  • Product name

    Anti-BOTOX antibody
    See all BOTOX primary antibodies
  • Description

    Rabbit polyclonal to BOTOX
  • Host species

    Rabbit
  • Specificity

    Clostridium botulinum Type A Neurotoxin.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length protein corresponding to BOTOX.

  • General notes

    Previously labelled as Clostridium botulinum A Toxoid.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: 2.68% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Toxin
    • Bacterial Toxin
    • Clostridium Toxin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/10000.
WB (1)
Use at an assay dependent dilution. Predicted molecular weight: 149 kDa.
Notes
ELISA
1/10000.
WB
Use at an assay dependent dilution. Predicted molecular weight: 149 kDa.

Target

  • Relevance

    Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.
  • Alternative names

    • Atx antibody
    • BotA antibody
    • BOTOX antibody
    • Botulinum neurotoxin type A antibody
    • Botulism A toxoid antibody
    • Clostridium botulinum A Toxoid antibody
    see all

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab20641? Please let us know so that we can cite the reference in this datasheet.

    ab20641 has been referenced in 4 publications.

    • Bagramyan K  et al. Substrates and controls for the quantitative detection of active botulinum neurotoxin in protease-containing samples. Anal Chem 85:5569-76 (2013). PubMed: 23656526
    • Scotcher MC  et al. Epitope characterization and variable region sequence of f1-40, a high-affinity monoclonal antibody to botulinum neurotoxin type a (Hall strain). PLoS ONE 4:e4924 (2009). WB . PubMed: 19290051
    • Scotcher MC  et al. Characterization of the epitope region of F1-2 and F1-5, two monoclonal antibodies to Botulinum neurotoxin type A. Hybridoma (Larchmt) 28:315-25 (2009). WB . PubMed: 19857112
    • Bagramyan K  et al. Attomolar detection of botulinum toxin type A in complex biological matrices. PLoS ONE 3:e2041 (2008). PubMed: 18446228

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Filter by Application

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    Western blot abreview for Anti-BOTOX antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Clostridium botulinum Purified protein (cell lysate)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    5 µg
    Specification
    cell lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. chung sei kim

    Verified customer

    Submitted Jun 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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