Overview

  • Product name

  • Description

    Rabbit polyclonal to BOTOX
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for Clostridium botulinum Type A Neurotoxin Light Chain.
  • Tested applications

    Suitable for: Dot blot, WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Other Immunogen Type corresponding to BOTOX.

  • General notes

    Previously labelled as Clostridium botulinum A Toxoid.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituents: 2.68% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 149 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.
  • Alternative names

    • Atx antibody
    • BotA antibody
    • BOTOX antibody
    • Botulinum neurotoxin type A antibody
    • Botulism A toxoid antibody
    • Clostridium botulinum A Toxoid antibody
    see all

References

ab20786 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Clostridium botulinum Purified protein (cell lysate)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
5 µg
Specification
cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. chung sei kim

Verified customer

Submitted Jun 20 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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