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    botox-antibody-ab20786.pdf

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Microbiology Toxin Bacterial Toxin Clostridium Toxin
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Anti-BOTOX antibody (ab20786)

  • Datasheet
  • SDS
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Abpromise

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Find out more.

Key features and details

  • Rabbit polyclonal to BOTOX
  • Suitable for: Dot blot, WB, ELISA
  • Reacts with: Species independent
  • Isotype: IgG

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Anti-Streptococcus pneumoniae antibody (ab20429)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-BOTOX antibody
    See all BOTOX primary antibodies
  • Description

    Rabbit polyclonal to BOTOX
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for Clostridium botulinum Type A Neurotoxin Light Chain.
  • Tested applications

    Suitable for: Dot blot, WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Other Immunogen Type corresponding to BOTOX.

  • General notes

    Previously labelled as Clostridium botulinum A Toxoid.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: 2.68% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Toxin
    • Bacterial Toxin
    • Clostridium Toxin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
Use at an assay dependent dilution.
WB (1)
Use at an assay dependent dilution. Predicted molecular weight: 149 kDa.
ELISA
Use at an assay dependent dilution.
Notes
Dot blot
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution. Predicted molecular weight: 149 kDa.
ELISA
Use at an assay dependent dilution.

Target

  • Relevance

    Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins. Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln-|-Arg-198 bond of SNAP-25.
  • Alternative names

    • Atx antibody
    • BotA antibody
    • BOTOX antibody
    • Botulinum neurotoxin type A antibody
    • Botulism A toxoid antibody
    • Clostridium botulinum A Toxoid antibody
    see all

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab20786? Please let us know so that we can cite the reference in this datasheet.

ab20786 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Filter by Application

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Western blot abreview for Anti-BOTOX antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Clostridium botulinum Purified protein (cell lysate)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
5 µg
Specification
cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Dr. chung sei kim

Verified customer

Submitted Jun 20 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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