Overview

  • Product name
  • Description
    Rabbit polyclonal to BOULE
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human, Monkey
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 53-266 of Human BOULE (Uniprot ID Q8N9W6)

  • Positive control
    • Mouse testis lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.

Target

  • Function
    Probable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation.
  • Tissue specificity
    Testis specific. Not expressed in early embryos, primordial germ cells and spermatogonial cells. First expressed in the cytoplasm of spermatocytes and then persists through meiosis.
  • Sequence similarities
    Belongs to the RRM DAZ family.
    Contains 1 DAZ-like domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bol (Drosophila boule homolog) like antibody
    • Bol antibody
    • Bol boule like antibody
    • Bol, boule like (Drosophila) antibody
    • bol, boule-like (Drosophila) antibody
    • Boll antibody
    • BOLL_HUMAN antibody
    • BOULE antibody
    • Boule like antibody
    • BOULE, Drosophila, homolog of antibody
    • Protein boule like antibody
    • Protein boule-like antibody
    • Putative uncharacterized protein BOLL antibody
    see all

Images

  • Anti-BOULE antibody (ab155261) at 1/1000 dilution + mouse testis lysate at 50 µg

    Predicted band size: 31 kDa



    12% SDS PAGE

References

ab155261 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cell (Testis- seminiferous tubules)
Permeabilization
No
Specification
Testis- seminiferous tubules
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 15 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate 0.05% Tween-20
Permeabilization
No
Specification
Testis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 29 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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