Overview

  • Product name

  • Description

    Rabbit polyclonal to BOULE/BOLL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Monkey
  • Immunogen

    Recombinant fragment corresponding to Human BOULE/BOLL aa 1-167.
    Database link: Q8N9W6

  • Positive control

    • WB: NT2D1 whole cell lysate. IHC-P: Human colon carcinoma tissue; mouse testis tissue.
  • General notes

     This product was previously labelled as BOULE

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Target

  • Function

    Probable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation.
  • Tissue specificity

    Testis specific. Not expressed in early embryos, primordial germ cells and spermatogonial cells. First expressed in the cytoplasm of spermatocytes and then persists through meiosis.
  • Sequence similarities

    Belongs to the RRM DAZ family.
    Contains 1 DAZ-like domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bol (Drosophila boule homolog) like antibody
    • Bol antibody
    • Bol boule like antibody
    • Bol, boule like (Drosophila) antibody
    • bol, boule-like (Drosophila) antibody
    • Boll antibody
    • BOLL_HUMAN antibody
    • BOULE antibody
    • Boule like antibody
    • BOULE, Drosophila, homolog of antibody
    • Protein boule like antibody
    • Protein boule-like antibody
    • Putative uncharacterized protein BOLL antibody
    see all

Images

  • Anti-BOULE/BOLL antibody (ab155027) at 1/500 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size: 31 kDa



    12 % SDS-PAGE
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling BOULE/BOLL with ab155027 at 1/250 dilution.

  • Paraffin-embedded mouse testis tissue labeling BOULE/BOLL using ab155027 at 1/500 dilution in immunohistochemical analysis.

    Antigen retrieval: EDTA-based (pH 8.0) 15 minutes.

References

ab155027 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155027.
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