Anti-BOULE/BOLL antibody (ab155027)
Key features and details
- Rabbit polyclonal to BOULE/BOLL
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BOULE/BOLL antibody
See all BOULE/BOLL primary antibodies -
Description
Rabbit polyclonal to BOULE/BOLL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Monkey -
Immunogen
Recombinant fragment corresponding to Human BOULE/BOLL aa 1-167.
Database link: Q8N9W6 -
Positive control
- WB: NT2D1 whole cell lysate. IHC-P: Human colon carcinoma tissue; mouse testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) |
Target
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Function
Probable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation. -
Tissue specificity
Testis specific. Not expressed in early embryos, primordial germ cells and spermatogonial cells. First expressed in the cytoplasm of spermatocytes and then persists through meiosis. -
Sequence similarities
Belongs to the RRM DAZ family.
Contains 1 DAZ-like domain.
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 66037 Human
- Entrez Gene: 75388 Mouse
- Omim: 606165 Human
- SwissProt: Q8N9W6 Human
- SwissProt: Q924M5 Mouse
- Unigene: 169797 Human
- Unigene: 483408 Mouse
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Alternative names
- Bol (Drosophila boule homolog) like antibody
- Bol antibody
- Bol boule like antibody
see all
Images
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Anti-BOULE/BOLL antibody (ab155027) at 1/500 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 31 kDa
12 % SDS-PAGE -
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling BOULE/BOLL with ab155027 at 1/250 dilution.
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Paraffin-embedded mouse testis tissue labeling BOULE/BOLL using ab155027 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: EDTA-based (pH 8.0) 15 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155027 has not yet been referenced specifically in any publications.