Overview

  • Product name

    Bovine Alpha 1 Acid Glycoprotein / AGP ELISA Kit
    See all Alpha 1 Acid Glycoprotein/AGP kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.706 ng/ml
  • Range

    3.125 ng/ml - 100 ng/ml
  • Recovery

    > 85 %

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 3.125ng - 100ng

  • Assay time

    1h 40m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Cow
  • Product overview

    Alpha 1 Acid Glycoprotein / AGP in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Alpha 1 Acid Glycoprotein / AGP protein in bovine biological fluids.

  • Notes

    In this assay the Alpha 1 Acid Glycoprotein / AGP present in samples reacts with the anti-Alpha 1 Acid Glycoprotein / AGP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alpha 1 Acid Glycoprotein / AGP antibodies conjugated with bovineradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1 Acid Glycoprotein / AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1 Acid Glycoprotein / AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1 Acid Glycoprotein / AGP in the test sample. The quantity of Alpha 1 Acid Glycoprotein / AGP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab205069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab205069 Bovine Alpha 1 Acid Glycoprotein / AGP ELISA kit.

Protocols

References

ab205069 has not yet been referenced specifically in any publications.

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