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Please check the attachment for the western result. my samples are whole cell lysate, using 8M Urea. Any suggestions?
Asked on Oct 17 2012
Thank you for your email.
Was the bovine heart mitochondrial lysate also prepared with 8M urea?
I am not sure if this is what the lab recommends for denaturing. I think regular loading buffer with 1% SDS would be recommended (Laemmli buffer).
I will check with them, but in the meantime please let me know as well.
Answered on Oct 17 2012