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Here is the new western result attached. Signal from COX-1 is much stronger, but big-size band is still here.
Thank you very much for providing all the information for me.
Asked on Nov 01 2012
Thank you for your reply.
The WB looks great now - with the bright band at around 37 kDa. The band at the higher MW is very faint and seems to be specific to your sample or sample preparation as the mitochondrial control lysate does not have this band.
I also looked at the images you had sent earlier. The blots from 2010 also showed a weak band at the higher MW. If the same samples were used, than that would confirm that this band is indeed related to your samples. The bovine mito samples do not show such band.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Answered on Nov 01 2012