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concerning my previous question about Abcam Bovine Heart Mitochondrial
lysate (ab110338), I would like to further ask the following:
- are mitochondria in this product intact?
- do the product also contain endoplasmic reticulum or other organelle
- for my studies I would need to know the composition of the buffer in
which Abcam Bovine Heart Mitochondrial lysate is (I need to reproduce
such buffer in order to run some reference measurements). Could it be
possible to know the reagents composition?
Thank you again for your help,
Asked on Feb 20 2013
Thank you for your enquiry and interest in our product.
I can confirm that the mitochondria preparation is whole mitochondria (intact) but because the mitochondria is frozen it is not coupled.
By this I mean you are not able to perform respiration experiments with either the clark electrode or the seahorse instrument.
The preparation is made from fresh bovine tissue so it is of fairly good quality. However, it may contain traces of other organelles such as peroxisomes, ER or cytoplasmic proteins.
If you need any further assistance in the future, please do not hesitate to contact me.
Answered on Feb 20 2013