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To determine the protein concentration of this lysate would I need to homogenise 1mg of this lysate in PBS or is it already a homogenised sample from which I can directly measure the protein concentration?
Asked on Oct 28 2013
Product ab110338 contains whole mitochondria in iso-osmotic buffer. There is no need to measure protein concentration of this product; the product is supplied at 5.5 mg/mL. Simply adjust the protein concentration with PBS to 5.3 mg/mL and add detergent according to the kit’s protocol.
If you wish to measure the protein concentration of ab110338, dilute an aliquot according the assay range (100-200x using Pierce Micro BCA) in 0.25% SDS, this will solubilise the mitochondrial membranes. Do not use this aliquot to perform the assay in kit ab109902. There is no need to homogenize the sample for BCA or for the kit’s assay.
Ariana VeigaAbcam Scientific Support
Answered on Oct 28 2013