Product nameBovine IgG ELISA kit - 45 minutes
See all IgG kits
Intra-assay Sample n Mean SD CV% 1/100 32 38µg/ml 6.5 % 1/200 32 44.17µg/ml 7.6 % 1/400 32 41.55µg/ml 10.8 % Inter-assay Sample n Mean SD CV% A 1/16 14 7.3µg/ml 5.87 % B 1/128 14 7.7µg/ml 5.89 % Bovine 1/100 16 1.2µg/ml 4.3 %
Range10 ng/ml - 2000 ng/ml
Assay durationOne step assay
The Bovine Immunoglobulin G ELISA Kit (ab273152) provides a rapid and easy method (one step ELISA) for the quantitative determination of bovine IgGs in cell culture supernatants and serums (CS, FCS, NBCS...) and contaminating bovine IgGs in batches of purified antibodies produced in vitro. The kit includes ready-to-use reagents necessary to analyze 89 samples in less than 45 minutes.
A polyclonal antibody specific to bovine IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and bovine IgG present in the sample are bound by the capture antibody. Then, an HRP (horseradish peroxidase) conjugated anti-bovine IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of bovine IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10 x 96 tests Bovine IgG standard 0.0 ng/ml 1 x 0.3ml 1 x 1ml Bovine IgG standard 1000 ng/ml 1 x 0.3ml 1 x 1ml Bovine IgG standard 125 ng/ml 1 x 0.3ml 1 x 1ml Bovine IgG standard 2000 ng/ml 1 x 0.3ml 1 x 1ml Bovine IgG standard 250 ng/ml 1 x 0.3ml 1 x 1ml Bovine IgG standard 31 ng/ml 1 x 0.3ml 1 x 1ml Bovine IgG standard 500 ng/ml 1 x 0.3ml 1 x 1ml Detection antibody 1 x 12ml 1 x 120ml Pre-coated microwells strips 1 x 96 tests 10 x 96 tests Sample Diluent 1 x 30ml 1 x 500ml Stop solution 1 x 12ml 1 x 120ml TMB Substrate 1 x 12ml 1 x 120ml
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
Our Abpromise guarantee covers the use of ab273152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab273152 has not yet been referenced specifically in any publications.