Key features and details
- Sensitivity: 1.245 ng/ml
- Range: 3.125 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
Product nameBovine IgG ELISA Kit
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10%
Sample typeCell culture supernatant, Milk, Serum, Plasma
Assay typeSandwich (quantitative)
Range3.125 ng/ml - 100 ng/ml
> 85 %
Sample specific recovery Sample type Average % Range Serum > 85 3.125ng/ml - 100ng/ml
Assay time0h 50m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Cow
Does not react with: Mouse, Rat, Rabbit, Human
IgG in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IgG protein in bovine cell culture supernatant, milk, plasma and serum.
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X HRP Conjugated Enzyme Antibody 1 x 150µl 20X Diluent Concentration 1 x 50ml 20X Wash Buffer Concentrate 1 x 50ml Anti-Bovine IgG ElISA Microplate 1 unit Bovine IgG Calibrator (Lyophilized) 1 vial Chromogen Substrate Solution 1 x 12ml Stop Solution 1 x 12ml
RelevanceImmunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer.
ab205078 has been referenced in 1 publication.
- Juniper DT et al. Bioefficacy of hydroxy-selenomethionine as a selenium supplement in pregnant dairy heifers and on the selenium status of their calves. J Dairy Sci 102:7000-7010 (2019). PubMed: 31155245