Key features and details
- Sensitivity: 16 pg/ml
- Range: 16.38 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
Product nameBovine IL-1 alpha ELISA Kit
See all IL-1 alpha kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range16.38 pg/ml - 4000 pg/ml
Sample specific recovery Sample type Average % Range Serum 94.68 86% - 104% Plasma 89.82 84% - 101% Cell culture media 112.6 108% - 119%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Cow
Bovine IL-1 alpha ELISA Kit (ab273196) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of bovine IL-1 alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for bovine IL-1 alpha coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- bovine IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Tested applicationsSuitable for: ELISAmore details
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Bovine IL-1 alpha coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Bovine IL-1 alpha Antibody 2 vials Bovine IL-1 alpha Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Sequence similaritiesBelongs to the IL-1 family.
DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
- Information by UniProt
- Hematopoietin 1
Our Abpromise guarantee covers the use of ab273196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab273196 has not yet been referenced specifically in any publications.