Key features and details
- Sensitivity: 0.43 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Product nameBovine IL-18 ELISA Kit
See all IL-18 kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.41 ng/ml - 100 ng/ml
Sample specific recovery Sample type Average % Range Serum 74.45 68% - 85% Plasma 70.23 69% - 72% Cell culture media 71.41 68% - 74%
Assay durationMultiple steps standard assay
Bovine IL-18 ELISA Kit (ab277387) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine IL-18 in serum (Bovine IL-18 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Bovine IL-18 coated on a 96-well plate. Standards and samples are pipetted into the wells and Bovine IL-18 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine IL-18 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Bovine IL-18 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Bovine IL-18 Antibody 2 vials Anti-Bovine IL-18 coated Microplate 1 unit Bovine IL-18 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionAugments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Sequence similaritiesBelongs to the IL-1 family.
- Information by UniProt
- IFN gamma inducing factor
- IFN-gamma-inducing factor
ab277387 has not yet been referenced specifically in any publications.