Key features and details
- Sensitivity: 8 pg/ml
- Range: 8 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow
Product nameBovine IL-4 ELISA Kit
See all IL-4 kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range8 pg/ml - 2000 pg/ml
Sample specific recovery Sample type Average % Range Serum 127.3 105% - 141% Plasma 120.3 101% - 140% Cell culture media 97.79 83% - 117%
Assay durationMultiple steps standard assay
Bovine IL-4 ELISA Kit (ab277388) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine IL-4 in serum (Bovine IL-4 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Bovine IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Bovine IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Bovine IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 150X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Bovine IL-4 Antibody 2 vials Anti-Bovine IL-4 coated Microplate 1 unit Bovine IL-4 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionParticipates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
Involvement in diseaseGenetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Sequence similaritiesBelongs to the IL-4/IL-13 family.
- Information by UniProt
- B cell growth factor 1
- B cell IgG differentiation factor
- B Cell Stimulatory Factor 1
ab277388 has not yet been referenced specifically in any publications.