Overview

  • Product name

    Bovine Interferon gamma ELISA Kit (IFNG)
    See all Interferon gamma kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.11 ng/ml
  • Range

    0.11 ng/ml - 30 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Plasma = 96.18 87% - 104%
    Cell culture media = 113.2 98% - 120%

  • Species reactivity

    Reacts with: Cow
  • Product overview

    Abcam’s IFN gamma bovine ELISA Kit (ab193681) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of bovine IFN gamma in serum, plasmas and cell culture supernatant. Plasma sample should be collected using EDTA or citrate as an anticoagulant. Heparin is not recommended for use in this assay.


    This assay employs an antibody specific for bovine IFN gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IFN gamma present in the samples. The wells are washed and biotinylated anti-bovine IFN gamma antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IFN gamma bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Bovine Interferon gamma in normal serum with ab193681, only in serum spiked with Bovine Interferon gamma.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    HRP-Streptavidin Concentrate (Item G) 1 x 200µl
    Biotinylated Bovine IFN-gamma Detection Antibody (lyophilized) 1 x 2 vials
    Bovine IFN-gamma Standards (lyophilized) 1 x 2 vials
    Pre Coated Bovine IFN-gamma Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity

    Released primarily from activated T lymphocytes.
  • Involvement in disease

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities

    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • IF 1
    • IFG
    • IFI
    • IFN gamma
    • IFN immune
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    • Type II Interferon
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example of typical bovine IFN gamma standard curve using Assay Diluent (used for serum/plasma standards).

Protocols

References

ab193681 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab193681.
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