Key features and details
- Sensitivity: 1.31 ng/ml
- Range: 1.31 ng/ml - 320 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Product nameBovine MCP1 ELISA Kit
See all MCP1 kits
Intra-assay Sample n Mean SD CV% overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range1.31 ng/ml - 320 ng/ml
Sample specific recovery Sample type Average % Range Serum 82.97 70% - 101% Plasma 86.95 73% - 103% Cell culture media 90.6 73% - 114%
Assay durationMultiple steps standard assay
Bovine MCP1 ELISA Kit (ab273192) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine MCP1 in serum (Bovine MCP1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Bovine MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Bovine MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Tested applicationsSuitable for: Sandwich ELISAmore details
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Bovine MCP1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Bovine MCP1 Antibody 2 vials Bovine MCP1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
- Information by UniProt
- C-C motif chemokine 2
Our Abpromise guarantee covers the use of ab273192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab273192 has not yet been referenced specifically in any publications.