Anti-Bovine MHC Class II DQ antibody [CC158] (Phycoerythrin) (ab129721)

Overview

  • Product name

    Anti-Bovine MHC Class II DQ antibody [CC158] (Phycoerythrin)
  • Description

    Mouse monoclonal [CC158] to Bovine MHC Class II DQ (Phycoerythrin)
  • Host species

    Mouse
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Cow
  • Positive control

    • Bovine Peripheral Blood lymphocytes

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 93% PBS, 5% Sucrose, 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    CC158
  • Myeloma

    NS1
  • Isotype

    IgG2a

Applications

Our Abpromise guarantee covers the use of ab129721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1. Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Target

  • Relevance

    Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • Cellular localization

    Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus › trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Database links

  • Alternative names

    • HLA-DQA1 antibody
    • DC-1 alpha chain antibody
    • DC-alpha antibody
    • HLA class II histocompatibility antigen, DQ alpha 1 chain antibody
    • HLA-DCA antibody
    • HLA-DQA1 antibody
    • MHC class II DQA1 antibody
    see all

Images

  • ab129721, undiluted staining Bovine MHC Class II DQ in Bovine Peripheral Blood lymphocytes by Flow Cytometry.

References

This product has been referenced in:

  • Yamakawa Y  et al. Identification and functional characterization of a bovine orthologue to DC-SIGN. J Leukoc Biol 83:1396-403 (2008). Read more (PubMed: 18319290) »
  • Van Rhijn I  et al. Massive, sustained gammadelta T cell migration from the bovine skin in vivo. J Leukoc Biol 81:968-73 (2007). Read more (PubMed: 17234682) »
See all 2 Publications for this product

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