Overview

  • Product name
    Anti-Bovine Papilloma Virus E2 antibody [1E4]
    See all Bovine Papilloma Virus E2 primary antibodies
  • Description
    Mouse monoclonal [1E4] to Bovine Papilloma Virus E2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    Mapped to amino acids 250-280.

  • Epitope
    Not Determined.

Properties

Applications

Our Abpromise guarantee covers the use of ab980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
  • Application notes
    IF (ICC): 1/500- 1/1000 (1hr, RT). Dilute in a diluent containing 1% BSA in PBS.
    IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
    WB: 1/1000 - 1/5000 (1hr, RT). Dilute in a diluent containing 2% non-fat dry milk in TBST.
    (Background can be reduced by adding NaCl up to a concentration of 1.5 M.)Incubate
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance
      The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
    • Cellular localization
      Host cell nucleus
    • Alternative names
      • BPVgp4 antibody
      • E2 antibody
      • Regulatory protein E2 antibody

    Images

    • Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
      Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
      All lanes : Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)

      Lane 1 : Purified recombinant Bovine Papillomavirus E2 protein at 15000 µg
      Lane 2 : Purified recombinant Bovine Papillomavirus E2 protein at 10000 µg
      Lane 3 : Purified recombinant Bovine Papillomavirus E2 protein at 5000 µg
      Lane 4 : Purified recombinant Bovine Papillomavirus E2 protein at 1000 µg

      Predicted band size: 45 kDa

    References

    This product has been referenced in:
    • Della Gatta G  et al. Reverse engineering of TLX oncogenic transcriptional networks identifies RUNX1 as tumor suppressor in T-ALL. Nat Med 18:436-40 (2012). ChIP . Read more (PubMed: 22366949) »
    • Kurg R  et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). Read more (PubMed: 10233926) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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