Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
- Datasheet
- References (2)
- Protocols
Overview
-
Product nameAnti-Bovine Papilloma Virus E2 antibody [1E4]
See all Bovine Papilloma Virus E2 primary antibodies -
DescriptionMouse monoclonal [1E4] to Bovine Papilloma Virus E2
-
Host speciesMouse
-
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
-
Species reactivityReacts with: Cow
-
Immunogen
Mapped to amino acids 250-280.
-
EpitopeNot Determined.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferConstituent: 50% Glycerol
-
Concentration information loading... -
PurityProtein A purified
-
ClonalityMonoclonal
-
Clone number1E4
-
IsotypeIgG1
-
Research areas
Associated products
-
Compatible Secondaries
-
Related Products
- Anti-Bovine Papilloma Virus E2 antibody [3E8] (ab1011)
- pQ-NTag/B vector (ab1041)
- pQ-Ctag/A vector (ab1051)
- pQM-NTag/C intron vector (ab1067)
- Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)
- Anti-E2 tag antibody [5E11] (ab977)
- Anti-E2 tag antibody [3F12] (ab978)
- Anti-Bovine Papilloma Virus E2 antibody [1E2] (ab979)
Applications
Our Abpromise guarantee covers the use of ab980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP | ||
| ICC/IF |
Application notes
IF (ICC): 1/500- 1/1000 (1hr, RT). Dilute in a diluent containing 1% BSA in PBS.
IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
WB: 1/1000 - 1/5000 (1hr, RT). Dilute in a diluent containing 2% non-fat dry milk in TBST.
(Background can be reduced by adding NaCl up to a concentration of 1.5 M.)Incubate
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
WB: 1/1000 - 1/5000 (1hr, RT). Dilute in a diluent containing 2% non-fat dry milk in TBST.
(Background can be reduced by adding NaCl up to a concentration of 1.5 M.)Incubate
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
RelevanceThe bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
-
Cellular localizationHost cell nucleus
-
Alternative names
- BPVgp4 antibody
- E2 antibody
- Regulatory protein E2 antibody
Images
-
![Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)](https://www.abcam.com/ps/products/0/ab980/Images/ab980-172809-ab980wb.jpg)
Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)All lanes : Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
Lane 1 : Purified recombinant Bovine Papillomavirus E2 protein at 15000 µg
Lane 2 : Purified recombinant Bovine Papillomavirus E2 protein at 10000 µg
Lane 3 : Purified recombinant Bovine Papillomavirus E2 protein at 5000 µg
Lane 4 : Purified recombinant Bovine Papillomavirus E2 protein at 1000 µg
Predicted band size: 45 kDa
Datasheets and documents
Protocols
References
This product has been referenced in:
- Della Gatta G et al. Reverse engineering of TLX oncogenic transcriptional networks identifies RUNX1 as tumor suppressor in T-ALL. Nat Med 18:436-40 (2012). ChIP . Read more (PubMed: 22366949) »
- Kurg R et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). Read more (PubMed: 10233926) »
