Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-Bovine Papilloma Virus E2 antibody [1E4]
See all Bovine Papilloma Virus E2 primary antibodies -
Description
Mouse monoclonal [1E4] to Bovine Papilloma Virus E2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Cow -
Immunogen
Mapped to amino acids 250-280.
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Epitope
Not Determined.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1E4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP | ||
| ICC/IF |
Application notes
IF (ICC): 1/500- 1/1000 (1hr, RT). Dilute in a diluent containing 1% BSA in PBS.
IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
WB: 1/1000 - 1/5000 (1hr, RT). Dilute in a diluent containing 2% non-fat dry milk in TBST.
(Background can be reduced by adding NaCl up to a concentration of 1.5 M.)Incubate
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution to isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.
WB: 1/1000 - 1/5000 (1hr, RT). Dilute in a diluent containing 2% non-fat dry milk in TBST.
(Background can be reduced by adding NaCl up to a concentration of 1.5 M.)Incubate
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication. -
Cellular localization
Host cell nucleus -
Alternative names
- BPVgp4 antibody
- E2 antibody
- Regulatory protein E2 antibody
Images
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![Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)](https://www.abcam.com/ps/products/0/ab980/Images/ab980-172809-anti-bovine-papilloma-virus-e2-antibody-1e4-western-blot.jpg)
Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)All lanes : Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
Lane 1 : Purified recombinant Bovine Papillomavirus E2 protein at 15000 µg
Lane 2 : Purified recombinant Bovine Papillomavirus E2 protein at 10000 µg
Lane 3 : Purified recombinant Bovine Papillomavirus E2 protein at 5000 µg
Lane 4 : Purified recombinant Bovine Papillomavirus E2 protein at 1000 µg
Predicted band size: 45 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Yamauchi T et al. FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells. Mol Cell Biol 37:N/A (2017). Read more (PubMed: 28069738) »
- Bocco BM et al. Type 2 Deiodinase Disruption in Astrocytes Results in Anxiety-Depressive-Like Behavior in Male Mice. Endocrinology 157:3682-95 (2016). Read more (PubMed: 27501182) »
