For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    bovine-papilloma-virus-e2-antibody-1e4-ab980.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Microbiology Interspecies Interaction Host Virus Interaction
Share by email

Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)

  • Datasheet
Submit a review Submit a question References (5)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)

    Key features and details

    • Mouse monoclonal [1E4] to Bovine Papilloma Virus E2
    • Suitable for: WB, IP, ICC/IF
    • Reacts with: Bovine papillomavirus
    • Isotype: IgG1

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Assay
    Product image
    Glucosylceramidase Activity Assay Kit (Fluorometric) (ab273339)

    View more associated products

    Overview

    • Product name

      Anti-Bovine Papilloma Virus E2 antibody [1E4]
      See all Bovine Papilloma Virus E2 primary antibodies
    • Description

      Mouse monoclonal [1E4] to Bovine Papilloma Virus E2
    • Host species

      Mouse
    • Tested applications

      Suitable for: WB, IP, ICC/IFmore details
    • Species reactivity

      Reacts with: Bovine papillomavirus
    • Immunogen

      Mapped to amino acids 250-280.

    • Epitope

      Not Determined.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.4
      Constituent: 50% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      1E4
    • Isotype

      IgG1
    • Research areas

      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Microbiology
      • Organism
      • Virus
      • DNA Virus
      • double stranded DNA Virus
      • Bovine Papilloma Virus

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Related Products

      • Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)
      • Anti-E2 tag antibody [5E11] (ab977)
      • Anti-E2 tag antibody [3F12] (ab978)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab980 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000 - 1/5000. Predicted molecular weight: 45 kDa.

    1hr, RT. Dilute in a diluent containing 2% non-fat dry milk in TBST. (Background can be reduced by adding NaCl up to a concentration of 1.5 M.)

    IP
    Use at an assay dependent concentration.

    To isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.

    ICC/IF
    1/500 - 1/1000.

    1hr, RT. Dilute in a diluent containing 1% BSA in PBS.

    Notes
    WB
    1/1000 - 1/5000. Predicted molecular weight: 45 kDa.

    1hr, RT. Dilute in a diluent containing 2% non-fat dry milk in TBST. (Background can be reduced by adding NaCl up to a concentration of 1.5 M.)

    IP
    Use at an assay dependent concentration.

    To isolate E2Tag-tagged proteins and associated proteins/protein binding partners. Use protein G-agarose.

    ICC/IF
    1/500 - 1/1000.

    1hr, RT. Dilute in a diluent containing 1% BSA in PBS.

    Target

    • Relevance

      The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
    • Cellular localization

      Host cell nucleus
    • Database links

      • SwissProt: P03122 Bovine papillomavirus
      • Alternative names

        • BPVgp4 antibody
        • E2 antibody
        • Regulatory protein E2 antibody

      Images

      • Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
        Western blot - Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)
        All lanes : Anti-Bovine Papilloma Virus E2 antibody [1E4] (ab980)

        Lane 1 : Purified recombinant Bovine Papillomavirus E2 protein at 15000 µg
        Lane 2 : Purified recombinant Bovine Papillomavirus E2 protein at 10000 µg
        Lane 3 : Purified recombinant Bovine Papillomavirus E2 protein at 5000 µg
        Lane 4 : Purified recombinant Bovine Papillomavirus E2 protein at 1000 µg

        Predicted band size: 45 kDa

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (5)

      Publishing research using ab980? Please let us know so that we can cite the reference in this datasheet.

      ab980 has been referenced in 5 publications.

      • Garcia-Diaz B  et al. Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling. Acta Neuropathol 138:457-476 (2019). PubMed: 31011859
      • Yamauchi T  et al. FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells. Mol Cell Biol 37:N/A (2017). PubMed: 28069738
      • Bocco BM  et al. Type 2 Deiodinase Disruption in Astrocytes Results in Anxiety-Depressive-Like Behavior in Male Mice. Endocrinology 157:3682-95 (2016). PubMed: 27501182
      • Della Gatta G  et al. Reverse engineering of TLX oncogenic transcriptional networks identifies RUNX1 as tumor suppressor in T-ALL. Nat Med 18:436-40 (2012). ChIP . PubMed: 22366949
      • Kurg R  et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). PubMed: 10233926

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab980.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2022 Abcam plc. All rights reserved.