Key features and details
- Mouse monoclonal [1H10] to Bovine Papilloma Virus E2
- Suitable for: WB, ICC/IF
- Isotype: IgG
Product nameAnti-Bovine Papilloma Virus E2 antibody [1H10]
See all Bovine Papilloma Virus E2 primary antibodies
DescriptionMouse monoclonal [1H10] to Bovine Papilloma Virus E2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Other species
Recombinant full length Bovine Papillomavirus E2-protein expressed in E Coli.
EpitopeMapped to amino acids 208-218 (RPDGVWVASEG)
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000 for 1 hour at RT, in TBST with 2% non-fat dry milk. Predicted molecular weight: 45 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
Cellular localizationHost cell nucleus
- Entrez Gene: 1489020 Other species
- BPVgp4 antibody
- E2 antibody
- Regulatory protein E2 antibody
All lanes : Anti-Bovine Papilloma Virus E2 antibody [1H10] (ab77174)
Lane 1 : Recombinant purified Bovine Papillomavirus E2 protein at 10000 µg
Lane 2 : Recombinant purified Bovine Papillomavirus E2 protein at 5000 µg
Lane 3 : Recombinant purified Bovine Papillomavirus E2 protein at 1000 µg
Lane 4 : Recombinant purified Bovine Papillomavirus E2 protein at 500 µg
Predicted band size: 45 kDa
ab77174 has been referenced in 2 publications.
- Kurg R et al. Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers. J Virol 80:11218-25 (2006). WB . PubMed: 16943289
- Kurg R et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). WB, ELISA . PubMed: 10233926