Overview

  • Product name

    Anti-Bovine Papilloma Virus E2 antibody [1H10]
    See all Bovine Papilloma Virus E2 primary antibodies
  • Description

    Mouse monoclonal [1H10] to Bovine Papilloma Virus E2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Recombinant full length Bovine Papillomavirus E2-protein expressed in E Coli.

  • Epitope

    Mapped to amino acids 208-218 (RPDGVWVASEG)

Properties

Applications

Our Abpromise guarantee covers the use of ab77174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: 1/1000 for 1 hour at RT, in PBS with 1% BSA.
    WB: 1/1000 - 1/5000 for 1 hour at RT, in TBST with 2% non-fat dry milk. Predicted molecular weight: 45 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
    • Cellular localization

      Host cell nucleus
    • Database links

      • Alternative names

        • BPVgp4 antibody
        • E2 antibody
        • Regulatory protein E2 antibody

      Images

      • All lanes : Anti-Bovine Papilloma Virus E2 antibody [1H10] (ab77174)

        Lane 1 : Recombinant purified Bovine Papillomavirus E2 protein at 10000 µg
        Lane 2 : Recombinant purified Bovine Papillomavirus E2 protein at 5000 µg
        Lane 3 : Recombinant purified Bovine Papillomavirus E2 protein at 1000 µg
        Lane 4 : Recombinant purified Bovine Papillomavirus E2 protein at 500 µg

        Predicted band size: 45 kDa

      References

      This product has been referenced in:

      • Kurg R  et al. Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers. J Virol 80:11218-25 (2006). WB . Read more (PubMed: 16943289) »
      • Kurg R  et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). WB, ELISA . Read more (PubMed: 10233926) »
      See all 2 Publications for this product

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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