Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)

Submit a review Q&A (1)
Immunocytochemistry/ Immunofluorescence - Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)

    Key features and details

    • Mouse monoclonal [5H4] to Bovine Papilloma Virus E2
    • Suitable for: ICC/IF, ELISA, IP
    • Isotype: IgG1

    Overview

    • Product name

      Anti-Bovine Papilloma Virus E2 antibody [5H4]
      See all Bovine Papilloma Virus E2 primary antibodies

    • Description

      Mouse monoclonal [5H4] to Bovine Papilloma Virus E2

    • Host species

      Mouse

    • Tested applications

      Suitable for: ICC/IF, ELISA, IPmore details
      Unsuitable for: WB

    • Species reactivity

      Reacts with: Other species

    • Epitope

      Conformational

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1072 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
    Use a concentration of 1 - 2 µg/ml.
    ELISA
    Use at an assay dependent dilution.
    IP
    Use at an assay dependent dilution.
    Notes
    ICC/IF
    Use a concentration of 1 - 2 µg/ml.
    ELISA
    Use at an assay dependent dilution.
    IP
    Use at an assay dependent dilution.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Relevance

      The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.

    • Cellular localization

      Host cell nucleus

    • Database links

      • Alternative names

        • BPVgp4 antibody
        • E2 antibody
        • Regulatory protein E2 antibody

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)
        Immunocytochemistry/ Immunofluorescence - Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)

        Immunofluorescent staining of U2OS cells expressing BPV E2 protein with ab1072. The cells were counter-stained with DAPI, and the upper shows an image taken with illumination at both 460 nm and 488 nm, whereas the lower image shows illumination at 488 nm.

      References (0)

      Publishing research using ab1072? Please let us know so that we can cite the reference in this datasheet.

      ab1072 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a question

      Question

      BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM multiple nonspecific bands with background. no specific bands revealed SAMPLE cell extracts from transient transfected 293T cells. E2-p300 fusion expression plasmids were transfected superfect was used as transfect reagents PRIMARY ANTIBODY E2 antibody (1:1000) ab1072, fresh shipment DETECTION METHOD ECL plus POSITIVE AND NEGATIVE CONTROLS USED positive control: 1) same cells were transfected with cyclin D1 and subsequent western blot was beautiful; 2)same cell lysate was bloted with another antibody, (anti-GDI), the results was nice and clean; our conclusion is that the E2 primary antibody does not work! ANTIBODY STORAGE CONDITIONS 4C SAMPLE PREPARATION standard tris buffer plus np-40, pmsf, dtt and protease inhibitors(tablet) AMOUNT OF PROTEIN LOADED 60ug ELECTROPHORESIS/GEL CONDITIONS 7% sds-page TRANSFER AND BLOCKING CONDITIONS semitry transfer, 5% milk in tpbs SECONDARY ANTIBODY anti-mouse-HRP conjugated HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES We are well experienced lab with many publications each year,and western blot is a routine in our lab. we have applied positive controls(for transfection, primary antibody, and 2nd antibody) for the western blot. E2 antibody did not pick up any specific signal other than many nonspecific bands. please ship another antibody that DO works in your hands, or please refund us, so we could buy E2 antibody from other sources.We are addressing reviewer's question for a paper, please understand that we are underpressure and we need to get the E2 western blot to work within 2-3 weeks. thank you.

      Read More
      Answer

      I'm sorry to hear you are having problems with ab1072. I am currently trying to find out more information about the protocol used to test the antibody in WB. Have you tested the blot without milk blocking and if the non specific bands could be due to the secondary antibody? How long did you incubate the primary antibody for? The absence of a band could be due to the levels of Bovine Papilloma Virus E2 being below WB detection levels, I am trying to find a positive control to help you and I apologise for the delay and appreciate your patience,

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com