Anti-Bovine Serum Albumin antibody [1C12] (ab79827)

Overview

  • Product name

    Anti-Bovine Serum Albumin antibody [1C12]
    See all Bovine Serum Albumin primary antibodies
  • Description

    Mouse monoclonal [1C12] to Bovine Serum Albumin
  • Host species

    Mouse
  • Specificity

    This antibody reacts specifically with BSA. It does not cross react with Human Serum Albumin or Albumin from other species.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Cow
    Does not react with: Rabbit, Human
  • Immunogen

    Bovine Serum Albumin.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    1C12
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/100000.
WB 1/2000 - 1/10000. Predicted molecular weight: 69 kDa.

Target

  • Information by UniProt
  • Database links

  • Alternative names

    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • Albumin antibody
    • BSA antibody
    • Cell growth inhibiting protein 42 antibody
    • Growth inhibiting protein 20 antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • Serum albumin antibody
    see all

Images

  • All lanes : Anti-Bovine Serum Albumin antibody [1C12] (ab79827) at 1/2000 dilution

    Lane 1 : Human Serum
    Lane 2 : Bovine Serum
    Lane 3 : Rabbit Serum

    Lysates/proteins at 10 µl per lane.

    Secondary
    All lanes : Goat anti-mouse IgG (H+L) peroxidase conjugate at 1/4000 dilution

    Predicted band size: 69 kDa
    Observed band size: 67 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Non-denaturing SDS-PAGE (7.5%).
  • Graph showing comparison of the reactivity of ab79287 with Bovine Serum Albumin, Ovalbumin, Rabbit Serum Albumin and Human Serum Albumin.

References

ab79827 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

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Answer

Thank you for contacting us. These two anti-BSA antibodies are provided as ascites. Monoclonal antibodies can be produced by growing hybridoma cells within the peritoneal cavity of a mouse. When injected into a mouse, the hybridoma cells multiply and produce fluid (ascites) in its abdomen. This fluid contains a high concentration of antibody which can be harvested. This usually provides higher antibody yields than hybridoma cell culture. Unfortunately, the concentration of the specific antibody cannot be determined as there are many proteins present in ascites. Furthermore, these antibodies are not further purified from the fluid harvested and thus no particular storage buffer is added. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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