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Dear technical support: This customer raised an enquiry about ab79827 (Anti-Bovine Serum Albumin antibody [1C12]) and ab3781 (Anti-Bovine Serum Albumin antibody [BSA-7G10]) He would likes to know the concentration and storage buffer of these antibodies. Could you please help this customer to solve these problems? Thanks for your kindly assistance.
Asked on Nov 23 2011
Thank you for contacting us. These two anti-BSA antibodies are provided as ascites. Monoclonal antibodies can be produced by growing hybridoma cells within the peritoneal cavity of a mouse. When injected into a mouse, the hybridoma cells multiply and produce fluid (ascites) in its abdomen. This fluid contains a high concentration of antibody which can be harvested. This usually provides higher antibody yields than hybridoma cell culture. Unfortunately, the concentration of the specific antibody cannot be determined as there are many proteins present in ascites. Furthermore, these antibodies are not further purified from the fluid harvested and thus no particular storage buffer is added. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Nov 23 2011