Overview

  • Product name
    Anti-Bovine Serum Albumin antibody [BSA-7G10]
    See all Bovine Serum Albumin primary antibodies
  • Description
    Mouse monoclonal [BSA-7G10] to Bovine Serum Albumin
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow
    Predicted to work with: Rabbit, Human
  • Immunogen

    Full length native protein (purified) (Cow).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituents: 1.2114% Tris, 0.7507% Glycine, 2% Sucrose
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    BSA-7G10
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/10000.
WB 1/1200 - 1/2000.

Target

References

This product has been referenced in:
  • Walkup WG  et al. Phosphorylation of synaptic GTPase-activating protein (synGAP) by Ca2+/calmodulin-dependent protein kinase II (CaMKII) and cyclin-dependent kinase 5 (CDK5) alters the ratio of its GAP activity toward Ras and Rap GTPases. J Biol Chem 290:4908-27 (2015). WB . Read more (PubMed: 25533468) »
  • Wagner CS  et al. Intracellular regulation of cross-presentation during dendritic cell maturation. PLoS One 8:e76801 (2013). Read more (PubMed: 24098562) »
See all 3 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (MEF)
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 15 2014

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (12% resolving, 4.8% stacking gel)
Sample
Rabbit Tissue lysate - whole (Heparinized rabbit blood)
Specification
Heparinized rabbit blood
Treatment
Rabbit blood digested by mosquitoes for 0 & 18hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 02 2014

Answer

Thank you for your reply.

I heard back from the lab that ab3781 can be used with this standard protocol:
- Blocking for 20 min to 1 hour with TBST 1X with 5% dry milk
- Wash briefly with TBST 1X
- Incubation with the antibody at a dilution between 1/1200 to 1/2000 for 1 to 4 hours at room temperature
- Wash with TBST 1X once briefly and 5 times for 5 min
Then incubate with the secondary antibody acourding manufacturer instructions.

I hope this information helps. If you are still experiencing problems with the antibody, please let me know and we would be happy to replace or refund the antibody for you.

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Answer

Thank you for your inquiry.

I heard back from the lab that this antibody has been successfully tested on BSA in WB. The blocking buffer was TBS-Tween 20 with 5% dry milk. Is this what you had tried?

What dilutions of the antibody have you tried? Plus were you incubating overnight at 4C?

I look forward to your reply.

Read More

Question
Answer

Thank you for contacting us.

Please type Bovine Serum Album in the search box or click on the following link

https://www.abcam.com/index.html?pageconfig=searchresults

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your phone call.
I am very sorry to hear that these antibodies did not work together in sandwich ELISA. I am sending you the following discount codes as compensation for trying the antibodies in an untested application. Unfortunately, our guarantee does not apply in this case.
Please let me know the other 2 anti-BSA antibodies that you would like to try together in sandwich ELISA and I'd be happy to issue you the testing discount codes as well.
DISCOUNT CODE: xxx for ab3781
DISCOUNT CODE: xxx for ab7637
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab3781 and ab7637 in sandwich ELISA. Each code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for sandwich ELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us. These two anti-BSA antibodies are provided as ascites. Monoclonal antibodies can be produced by growing hybridoma cells within the peritoneal cavity of a mouse. When injected into a mouse, the hybridoma cells multiply and produce fluid (ascites) in its abdomen. This fluid contains a high concentration of antibody which can be harvested. This usually provides higher antibody yields than hybridoma cell culture. Unfortunately, the concentration of the specific antibody cannot be determined as there are many proteins present in ascites. Furthermore, these antibodies are not further purified from the fluid harvested and thus no particular storage buffer is added. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry. Unfortunately, epitope mapping was not done on this product, therefore we do not have any information on the exact epitope binding region. I am sorry I can't be of more assistance in this occasion but if there is anything else that I can help you with, please do not hesitate to contact me.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Purified protein (brain)
Loading amount
10 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Oct 16 2006

Question
Answer

This antibody is only available in liquid form.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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