Recombinant Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12774] to Bovine Serum Albumin
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Cow, Human
Overview
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Product name
Anti-Bovine Serum Albumin antibody [EPR12774]
See all Bovine Serum Albumin primary antibodies -
Description
Rabbit monoclonal [EPR12774] to Bovine Serum Albumin -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P CowWB MouseCowHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, Human plasma, human serum, HeLa whole cell lysate (ab150035), bovine serum albumin, mouse kidney and spleen, rat kidney, spleen and brain, Raw 264.7, PC12 cell lysates, Human liver tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12774 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Hep G2 whole cell lysate (ab166833)
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- NIH/3T3 whole cell lysate (ab7179)
- RAW 264.7 whole cell lysate (ab7187)
- Mouse spleen tissue lysate - total protein (ab7937)
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Cow
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WB |
Mouse
Cow
Human
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Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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Notes |
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IHC-P
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
Target
- Information by UniProt
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Database links
- Entrez Gene: 280717 Cow
- Entrez Gene: 213 Human
- Entrez Gene: 11657 Mouse
- Entrez Gene: 24186 Rat
- Omim: 103600 Human
- SwissProt: P02769 Cow
- SwissProt: P02768 Human
- SwissProt: P07724 Mouse
see all -
Alternative names
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Bovine liver labeling Serum albumin with ab192603 at 1/300 dilution. A prediluted HRP Polymer Rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin. Negative control also shown.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/1000 dilution
Lane 1 : Mouse kidney
Lane 2 : Mouse spleen
Lane 3 : Rat brain
Lane 4 : Rat kidney
Lane 5 : Rat spleen
Lane 6 : Raw264.7 cell lysate
Lane 7 : PC-12 cell lysate
Lane 8 : NIH-3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa -
Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/1000 dilution + Bovine Serum albumin at 0.001 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
Predicted band size: 69 kDa
Observed band size: 69 kDa -
Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/10000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa -
All lanes : Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human plasma
Lane 3 : Human serum
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab192603 has been referenced in 4 publications.
- Ye Z et al. Mesenchymal stem cells overexpressing hepatocyte nuclear factor-4 alpha alleviate liver injury by modulating anti-inflammatory functions in mice. Stem Cell Res Ther 10:149 (2019). PubMed: 31133062
- Yudasaka M et al. Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes. Sci Rep 8:14446 (2018). PubMed: 30262832
- Yudasaka M et al. Near-Infrared Photoluminescent Carbon Nanotubes for Imaging of Brown Fat. Sci Rep 7:44760 (2017). PubMed: 28317858
- Liu Y et al. S100P is associated with proliferation and migration in nasopharyngeal carcinoma. Oncol Lett 14:525-532 (2017). PubMed: 28693201