RecombinantRabMAb

Recombinant Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)

Submit a review Submit a question References (4)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
  • Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12774] to Bovine Serum Albumin
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Cow, Human

Overview

  • Product name

    Anti-Bovine Serum Albumin antibody [EPR12774]
    See all Bovine Serum Albumin primary antibodies

  • Description

    Rabbit monoclonal [EPR12774] to Bovine Serum Albumin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Cow
    WB
    Mouse
    Cow
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, Human plasma, human serum, HeLa whole cell lysate (ab150035), bovine serum albumin, mouse kidney and spleen, rat kidney, spleen and brain, Raw 264.7, PC12 cell lysates, Human liver tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12774

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab192603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Cow
WB
Mouse
Cow
Human
Application Abreviews Notes
IHC-P
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
Notes
IHC-P
1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Bovine liver labeling Serum albumin with ab192603 at 1/300 dilution.  A prediluted HRP Polymer Rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    All lanes : Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/1000 dilution

    Lane 1 : Mouse kidney
    Lane 2 : Mouse spleen
    Lane 3 : Rat brain
    Lane 4 : Rat kidney
    Lane 5 : Rat spleen
    Lane 6 : Raw264.7 cell lysate
    Lane 7 : PC-12 cell lysate
    Lane 8 : NIH-3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/1000 dilution + Bovine Serum albumin at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    All lanes : Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human plasma
    Lane 3 : Human serum

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)

References (4)

Publishing research using ab192603? Please let us know so that we can cite the reference in this datasheet.

ab192603 has been referenced in 4 publications.

  • Ye Z  et al. Mesenchymal stem cells overexpressing hepatocyte nuclear factor-4 alpha alleviate liver injury by modulating anti-inflammatory functions in mice. Stem Cell Res Ther 10:149 (2019). PubMed: 31133062
  • Yudasaka M  et al. Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes. Sci Rep 8:14446 (2018). PubMed: 30262832
  • Yudasaka M  et al. Near-Infrared Photoluminescent Carbon Nanotubes for Imaging of Brown Fat. Sci Rep 7:44760 (2017). PubMed: 28317858
  • Liu Y  et al. S100P is associated with proliferation and migration in nasopharyngeal carcinoma. Oncol Lett 14:525-532 (2017). PubMed: 28693201

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab192603.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com