Recombinant
RabMAb

Recombinant Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)

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Overview

  • Product name

    Anti-Bovine Serum Albumin antibody [EPR12774]
    See all Bovine Serum Albumin primary antibodies

  • Description

    Rabbit monoclonal [EPR12774] to Bovine Serum Albumin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human

  • Immunogen

    Synthetic peptide within Bovine Serum Albumin aa 150-250. The exact sequence is proprietary.
    Database link: P02769

  • Positive control

    • HepG2, Human plasma, human serum, HeLa whole cell lysate (ab150035), bovine serum albumin, mouse kidney and spleen, rat kidney, spleen and brain, Raw 264.7, PC12 cell lysates, Human liver tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12774

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Bovine liver labeling Serum albumin with ab192603 at 1/300 dilution.  A prediluted HRP Polymer Rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    All lanes : Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/1000 dilution

    Lane 1 : Mouse kidney
    Lane 2 : Mouse spleen
    Lane 3 : Rat brain
    Lane 4 : Rat kidney
    Lane 5 : Rat spleen
    Lane 6 : Raw264.7 cell lysate
    Lane 7 : PC-12 cell lysate
    Lane 8 : NIH-3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/1000 dilution + Bovine Serum albumin at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    Western blot - Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603)
    All lanes : Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human plasma
    Lane 3 : Human serum

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa

References

This product has been referenced in:

  • Ye Z  et al. Mesenchymal stem cells overexpressing hepatocyte nuclear factor-4 alpha alleviate liver injury by modulating anti-inflammatory functions in mice. Stem Cell Res Ther 10:149 (2019). Read more (PubMed: 31133062) »
  • Yudasaka M  et al. Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes. Sci Rep 8:14446 (2018). Read more (PubMed: 30262832) »
See all 4 Publications for this product

Publishing research using ab192603? Please let us know so that we can cite the reference in this datasheet.

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