Key features and details
- Rabbit polyclonal to BPGM
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BPGM antibody
See all BPGM primary antibodies
DescriptionRabbit polyclonal to BPGM
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow
Recombinant fragment containing a sequence corresponding to a region within amino acids 1-216 of Human BPGM (NP_001715).
- 293T, A431 and H1299 whole cell lysates; HeLa cells; HeLaS3, Molt-4 and Raji cell lines
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 30 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionPlays a major role in regulating hemoglobin oxygen affinity by controlling the levels of 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 22.214.171.124) and phosphatase (EC 126.96.36.199) activities.
Involvement in diseaseDefects in BPGM are the cause of bisphosphoglycerate mutase deficiency (BPGMD) [MIM:222800]. A disease characterized by hemolytic anemia, splenomegaly, cholelithiasis and cholecystitis.
Sequence similaritiesBelongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
modificationsGlycation of Lys-159 in diabetic patients inactivates the enzyme.
- Information by UniProt
- 2 antibody
- 2,3-bisphosphoglycerate mutase antibody
- 2,3-bisphosphoglycerate mutase, erythrocyte antibody
All lanes : Anti-BPGM antibody (ab97497) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 30 kDa
ab97497, at 1/100 dilution, staining BPGM in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
ab97497 has not yet been referenced specifically in any publications.