Recombinant Anti-BPGM antibody [EPR15127] (ab186434)
![Western blot - Anti-BPGM antibody [EPR15127] (ab186434)](https://www.abcam.com/ps/products/186/ab186434/Images/ab186434-220688-anti-bpgm-antibody-epr15127-western-blot.jpg "Western blot - Anti-BPGM antibody [EPR15127] (ab186434)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15127] to BPGM
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BPGM antibody [EPR15127]
See all BPGM primary antibodies -
Description
Rabbit monoclonal [EPR15127] to BPGM -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human placenta and blood lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15127 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities. -
Involvement in disease
Defects in BPGM are the cause of bisphosphoglycerate mutase deficiency (BPGMD) [MIM:222800]. A disease characterized by hemolytic anemia, splenomegaly, cholelithiasis and cholecystitis. -
Sequence similarities
Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily. -
Post-translational
modificationsGlycation of Lys-159 in diabetic patients inactivates the enzyme. - Information by UniProt
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Database links
- Entrez Gene: 669 Human
- Omim: 613896 Human
- SwissProt: P07738 Human
- Unigene: 198365 Human
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Alternative names
- 2 antibody
- 2,3-bisphosphoglycerate mutase antibody
- 2,3-bisphosphoglycerate mutase, erythrocyte antibody
see all
Images
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Western blot - Anti-BPGM antibody [EPR15127] (ab186434)Anti-BPGM antibody [EPR15127] (ab186434) at 1/1000 dilution + Human placenta lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa -
![Western blot - Anti-BPGM antibody [EPR15127] (ab186434)](https://www.abcam.com/ps/products/186/ab186434/Images/ab186434-220689-anti-bpgm-antibody-epr15127-western-blot.jpg)
Western blot - Anti-BPGM antibody [EPR15127] (ab186434)Anti-BPGM antibody [EPR15127] (ab186434) at 1/1000 dilution + Human blood lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa -
![Anti-BPGM antibody [EPR15127] (ab186434)](https://www.abcam.com/ps/products/186/ab186434/Images/ab186434-3-benefits-of-recombinant-antibodies.png)
Anti-BPGM antibody [EPR15127] (ab186434)
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab186434 has not yet been referenced specifically in any publications.
