Overview

  • Product name

    Anti-BPGM antibody [EPR15128]
    See all BPGM primary antibodies
  • Description

    Rabbit monoclonal [EPR15128] to BPGM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human BPGM aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P07738

  • Positive control

    • Human placenta lysate, Human blood lysate; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab186739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
Flow Cyt 1/110.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/50 - 1/70.

Target

  • Function

    Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities.
  • Involvement in disease

    Defects in BPGM are the cause of bisphosphoglycerate mutase deficiency (BPGMD) [MIM:222800]. A disease characterized by hemolytic anemia, splenomegaly, cholelithiasis and cholecystitis.
  • Sequence similarities

    Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
  • Post-translational
    modifications

    Glycation of Lys-159 in diabetic patients inactivates the enzyme.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2 antibody
    • 2,3-bisphosphoglycerate mutase antibody
    • 2,3-bisphosphoglycerate mutase, erythrocyte antibody
    • 2,3-bisphosphoglycerate synthase antibody
    • 2,3-diphosphoglycerate mutase antibody
    • 3-bisphosphoglycerate mutase antibody
    • 3-bisphosphoglycerate synthase antibody
    • 3-diphosphoglycerate mutase antibody
    • Ab2 098 antibody
    • AI323730 antibody
    • AL022789 antibody
    • Bisphosphoglycerate mutase antibody
    • BPG dependent PGAM antibody
    • BPG-dependent PGAM antibody
    • Bpgm antibody
    • C86192 antibody
    • DPGM antibody
    • Erythrocyte 2,3 bisphosphoglycerate mutase antibody
    • erythrocyte antibody
    • PMGE_HUMAN antibody
    see all

Images

  • Anti-BPGM antibody [EPR15128] (ab186739) at 1/10000 dilution + Human blood lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Western blot analysis on immunoprecipitation from 1) Human blood lysate and 2) PBS, labeling BPGM using 186739 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

  • Anti-BPGM antibody [EPR15128] (ab186739) at 1/1000 dilution + Human placenta lysate at 20 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling BPGM with ab186739 at a 1/110 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

References

ab186739 has not yet been referenced specifically in any publications.

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