Recombinant
RabMAb

Anti-BPI antibody [EPR12350(B)] (ab175231)

Overview

  • Product name
    Anti-BPI antibody [EPR12350(B)]
  • Description
    Rabbit monoclonal [EPR12350(B)] to BPI
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human BPI aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: P17213

  • Positive control
    • Human bone marrow, THP1 and HL60 lysates; HL60 cells; THP1 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab175231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Relevance
      The antimicrobial protein Bactericidal Permeability Increasing protein (BPI) is a 55 kDa protein found in the primary granules of polymorhonuclear leukocytes (PMN). The cytotoxicity action of BPI is limited to gram negative bacteria, reflecting the high affinity of BPI for bacterial LPS. Binding of BPI to live bacteria via LPS causes anti-infective activites: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils.
    • Cellular localization
      Cell Membrane and Secreted. Membrane associated in polymorphonuclear Leukocytes (PMN) granules.
    • Database links
    • Alternative names
      • Bactericidal permeability-increasing protein antibody
      • bactericidal/permeability-increasing protein antibody
      • BPI antibody
      • BPI fold containing family D, member 1 antibody
      • BPIFD1 antibody
      • CAP 57 antibody
      • rBPI antibody
      • recombinant BPI holoprotein, rBPI antibody
      see all

    Images

    • Immunofluorescent analysis of HL60 cells labeling BPI with ab175231 at 1/100 (red) and DAPI staining (blue).

    • All lanes : Anti-BPI antibody [EPR12350(B)] (ab175231) at 1/1000 dilution

      Lane 1 : Human bone marrow lysate
      Lane 2 : THP1 lysate
      Lane 3 : HL60 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 54 kDa
      Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.

    • Flow Cytometrical analysis of permeabilized THP1 cells labeling BPI with ab175231 antibody at 1/100 (red) or a rabbit IgG (green).

    References

    ab175231 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab175231.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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