Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12350(B)] to BPI
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Product nameAnti-BPI antibody [EPR12350(B)]
See all BPI primary antibodies
DescriptionRabbit monoclonal [EPR12350(B)] to BPI
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human BPI aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: P17213
- Human bone marrow, THP1 and HL60 lysates; HL60 cells; THP1 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 54 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceThe antimicrobial protein Bactericidal Permeability Increasing protein (BPI) is a 55 kDa protein found in the primary granules of polymorhonuclear leukocytes (PMN). The cytotoxicity action of BPI is limited to gram negative bacteria, reflecting the high affinity of BPI for bacterial LPS. Binding of BPI to live bacteria via LPS causes anti-infective activites: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils.
Cellular localizationCell Membrane and Secreted. Membrane associated in polymorphonuclear Leukocytes (PMN) granules.
- Bactericidal permeability-increasing protein antibody
- bactericidal/permeability-increasing protein antibody
- BPI antibody
Immunofluorescent analysis of HL60 cells labeling BPI with ab175231 at 1/100 (red) and DAPI staining (blue).
All lanes : Anti-BPI antibody [EPR12350(B)] (ab175231) at 1/1000 dilution
Lane 1 : Human bone marrow lysate
Lane 2 : THP1 lysate
Lane 3 : HL60 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.
Flow Cytometrical analysis of permeabilized THP1 cells labeling BPI with ab175231 antibody at 1/100 (red) or a rabbit IgG (green).
ab175231 has not yet been referenced specifically in any publications.