Product nameAnti-BPI antibody [EPR12350(B)]
DescriptionRabbit monoclonal [EPR12350(B)] to BPI
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human BPI aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: P17213
- Human bone marrow, THP1 and HL60 lysates; HL60 cells; THP1 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 54 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceThe antimicrobial protein Bactericidal Permeability Increasing protein (BPI) is a 55 kDa protein found in the primary granules of polymorhonuclear leukocytes (PMN). The cytotoxicity action of BPI is limited to gram negative bacteria, reflecting the high affinity of BPI for bacterial LPS. Binding of BPI to live bacteria via LPS causes anti-infective activites: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils.
Cellular localizationCell Membrane and Secreted. Membrane associated in polymorphonuclear Leukocytes (PMN) granules.
- Bactericidal permeability-increasing protein antibody
- bactericidal/permeability-increasing protein antibody
- BPI antibody
Immunofluorescent analysis of HL60 cells labeling BPI with ab175231 at 1/100 (red) and DAPI staining (blue).
All lanes : Anti-BPI antibody [EPR12350(B)] (ab175231) at 1/1000 dilution
Lane 1 : Human bone marrow lysate
Lane 2 : THP1 lysate
Lane 3 : HL60 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands.
Flow Cytometrical analysis of permeabilized THP1 cells labeling BPI with ab175231 antibody at 1/100 (red) or a rabbit IgG (green).
ab175231 has not yet been referenced specifically in any publications.