Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferTris saline, 0.02% Na azide, pH7.3 with 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab1077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 24076025|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).|
FunctionMay act as a mediator of the PTEN growth-suppressive signaling pathway. May play a role in developmental processes.
Tissue specificityUbiquitously expressed in all fetal tissues examined including heart, brain, liver, and kidney. Also expressed at lower levels in both adult heart and hypertrophic heart.
Sequence similaritiesContains 2 ANK repeats.
Contains 2 BTB (POZ) domains.
- Information by UniProt
- ABTB 1 antibody
- Abtb1 antibody
- ABTB1_HUMAN antibody
ab1077 at a concentration of 0.2ab1077 at a concentration of 0.2µg/ml staining approximately 40kDa BPOZ in human brain extract (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
µg/ml staining approximately 40kDa BPOZ in human brain extract (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunocytochemical/Immunofluorescence analysis of mouse primary neurons stimulated with 5 μM of MPP+ for 0, 6, 12, and 24 hours labelling BPOZ (green), TH (red) and Nuclei (DAPI - blue).
Coverslips containing neurons cultured to 70–80% confluence were fixed with chilled methanol overnight, followed by two rinses with PBS. Samples were blocked with 2% BSA in PBS containing Tween 20 and Triton X-100 for 30 minutes and incubated at room temperature under shaking conditions for 6 hours in PBS containing the ab1077 (1:200), TH (1:1000). After four 15 minute washes in PBS, slides were incubated with Cy2- or Cy5-labelled secondary antibodies (1:200) for 1 hour under shaking conditions. Following four 15 minute washes with PBS, cells were incubated for 4–5 minutes with DAPI (1:10000).
This product has been referenced in:
- Roy A & Pahan K Ankyrin repeat and BTB/POZ domain containing protein-2 inhibits the aggregation of alpha-synuclein: Implications for Parkinson's disease. FEBS Lett 587:3567-74 (2013). ICC/IF ; Mouse . Read more (PubMed: 24076025) »