Overview

  • Product name

    Anti-BPOZ/ABTB1 antibody
  • Description

    Goat polyclonal to BPOZ/ABTB1
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human BPOZ/ABTB1 aa 323-336 (N terminal).
    Sequence:

    RALEDLLVSIGLDC

  • Positive control

    • Heart lysate.
  • General notes

     This product was previously labelled as BPOZ

     

Properties

Applications

Our Abpromise guarantee covers the use of ab1077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24076025
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    May act as a mediator of the PTEN growth-suppressive signaling pathway. May play a role in developmental processes.
  • Tissue specificity

    Ubiquitously expressed in all fetal tissues examined including heart, brain, liver, and kidney. Also expressed at lower levels in both adult heart and hypertrophic heart.
  • Sequence similarities

    Contains 2 ANK repeats.
    Contains 2 BTB (POZ) domains.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABTB 1 antibody
    • Abtb1 antibody
    • ABTB1_HUMAN antibody
    • Ankyrin repeat and BTB (POZ) domain containing 1 antibody
    • Ankyrin repeat and BTB/POZ domain containing 1 antibody
    • Ankyrin repeat and BTB/POZ domain containing protein 1 antibody
    • Ankyrin repeat and BTB/POZ domain-containing protein 1 antibody
    • EF1ABP antibody
    • Elongation factor 1A binding protein antibody
    • Elongation factor 1A-binding protein antibody
    • MGC20585 antibody
    • PP 2259 antibody
    • PP2259 antibody
    see all

Images

  • ab1077 at a concentration of 0.2µg/ml staining approximately 40kDa  BPOZ/ABTB1 in human brain extract (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    ab1077 at a concentration of 0.2µg/ml staining approximately 40kDa BPOZ/ABTB1 in human brain extract (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

  • Immunocytochemical/Immunofluorescence analysis of mouse primary neurons stimulated with 5 μM of MPP+ for 0, 6, 12, and 24 hours labelling BPOZ/ABTB1 (green), TH (red) and Nuclei (DAPI - blue). 

    Coverslips containing neurons cultured to 70–80% confluence were fixed with chilled methanol overnight, followed by two rinses with PBS. Samples were blocked with 2% BSA in PBS containing Tween 20 and Triton X-100 for 30 minutes and incubated at room temperature under shaking conditions for 6 hours in PBS containing the ab1077 (1:200), TH (1:1000). After four 15 minute washes in PBS, slides were incubated with Cy2- or Cy5-labelled secondary antibodies (1:200) for 1 hour under shaking conditions. Following four 15 minute washes with PBS, cells were incubated for 4–5 minutes with DAPI (1:10000).

References

This product has been referenced in:

  • Roy A & Pahan K Ankyrin repeat and BTB/POZ domain containing protein-2 inhibits the aggregation of alpha-synuclein: Implications for Parkinson's disease. FEBS Lett 587:3567-74 (2013). ICC/IF ; Mouse . Read more (PubMed: 24076025) »
See 1 Publication for this product

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