Product nameAnti-BPR antibody [EPR5201]
See all BPR primary antibodies
DescriptionRabbit monoclonal [EPR5201] to BPR
Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human BPR aa 250 to the C-terminus. The exact sequence is proprietary.
- U87-MG, HT-1376, MCF7, and PC-3 cell lysates; Human breast carcinoma tissue IF/ICC: HeLa cell line.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as BCL2L12
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 37 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Tissue specificityExpressed mainly in breast, thymus, prostate, fetal liver, colon, placenta, pancreas, small intestine, spinal cord, kidney, and bone marrow and to a lesser extent in many other tissues. Isoform 2 is primarily expressed in skeletal muscle.
Sequence similaritiesBelongs to the Bcl-2 family.
- Information by UniProt
- B2L12_HUMAN antibody
- BCL 2 like 12 (proline rich) antibody
- BCL 2 like 12 antibody
All lanes : Anti-BPR antibody [EPR5201] (ab108346) at 1/1000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : HT-1376 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : PC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
ICC/IF image of ab108346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108346, 1/100) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
Overlay histogram showing HeLa cells stained with ab108346 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108346, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemical analysis of BPR in paraffin embedded Human breast carcinoma tissue, using ab108346 at a dilution of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.