Recombinant
RabMAb

Recombinant Anti-BPR antibody [EPR5201] (ab108346)

Overview

  • Product name

    Anti-BPR antibody [EPR5201]
    See all BPR primary antibodies
  • Description

    Rabbit monoclonal [EPR5201] to BPR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BPR aa 250 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • U87-MG, HT-1376, MCF7, and PC-3 cell lysates; Human breast carcinoma tissue IF/ICC: HeLa cell line.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as BCL2L12

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 37 kDa).
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.

Target

  • Tissue specificity

    Expressed mainly in breast, thymus, prostate, fetal liver, colon, placenta, pancreas, small intestine, spinal cord, kidney, and bone marrow and to a lesser extent in many other tissues. Isoform 2 is primarily expressed in skeletal muscle.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • B2L12_HUMAN antibody
    • BCL 2 like 12 (proline rich) antibody
    • BCL 2 like 12 antibody
    • BCL 2 like 12 isoform 1 antibody
    • Bcl 2 like 12 protein antibody
    • Bcl 2 related proline rich protein antibody
    • Bcl-2-like protein 12 antibody
    • Bcl-2-related proline-rich protein antibody
    • BCL2 like 12 (proline rich) antibody
    • BCL2 like 12 antibody
    • BCL2 like 12 isoform 1 antibody
    • Bcl2 like 12 protein antibody
    • Bcl2 related proline rich protein antibody
    • Bcl2-L-12 antibody
    • BCL2L12 antibody
    • BPR antibody
    • MGC120313 antibody
    • MGC120314 antibody
    • MGC120315 antibody
    see all

Images

  • All lanes : Anti-BPR antibody [EPR5201] (ab108346) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : HT-1376 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : PC-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 37 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab108346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108346, 1/100) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Overlay histogram showing HeLa cells stained with ab108346 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108346, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunohistochemical analysis of BPR in paraffin embedded Human breast carcinoma tissue, using ab108346 at a dilution of 1/500.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Lu W  et al. Long Noncoding RNA LOC100129973 Suppresses Apoptosis by Targeting miR-4707-5p and miR-4767 in Vascular Endothelial Cells. Sci Rep 6:21620 (2016). WB . Read more (PubMed: 26887505) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Jul 10 2014

Answer

You're welcome!

I wish you the best of luck with your research in the future. Please do not hesitate to contact me if I can be of further assistance.

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Answer

Thank you for your reply and the requested information.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question

I have been a loyal customer of Abcam for quite some time, having purchased >50 antibodies from the company over the past five years alone. Typically, I have found Abcam antibodies to be of supreme quality, and they have tended to perform very well in their recommended applications when I follow the guidelines outlined on their spec sheets.
It is for this reason that I was surprised and disappointed by the poor performance of three different antibodies that I have purchased from Abcam over the past year:
ab108346 (BCL2L12)
ab115467 (Bcl-G)
ab94429 (BIRC6)
Each of these three antibodies are recommended for use in IHC from paraffin sections for detection of the corresponding human proteins. However, despite the fact that I adhered strictly to the guidelines and dilutions outlined on each of their spec sheets, I failed to detect appropriate signal from any of these three antibodies from paraffin-embedded human tumor samples that I know express these three proteins based on independent validation methods. In these same experiments, I did observe positive signal from other antibodies, suggesting that my secondary antibodies and other reagents, as well as antigen retrieval, are not to blame. Moreover, I have since purchased IHC antibodies against several of these proteins from other vendors and have obtained more satisfactory staining with their reagents on these same tumor specimens, again verifying their expression in these samples. Together, these facts lead me to conclude that the problem arose with these three primary antibodies themselves.
Again, I have been a loyal customer of Abcam for many years, but these experiences have left me disappointed and I therefore wished to bring them to your attention as I contemplate where I will purchase my antibodies from in the future.

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Answer

Thank you for contacting Abcam.

I am sorry that you experienced difficulties with several of our antibodies in your experiments. I have reviewed the product datasheets and both ab108346 and ab115467 are validated and guaranteed to work in IHC-P with human tissue, therefore I am happy to offer a replacement or refund for these products. It appears that ab94429 has only been validated for WB. Since we have not tested the product in IHC-P we are not able to guarantee that it will work in this application.

In order to process your request, would you please provide the following information:
- order number or PO
- brief summary of your protocol (we will further investigate the problem you have reported and this will help us to do so)
- your preference of receiving a replacement (and which products you would like to receive) or a refund

I look forward to your reply so that I may assist you further and I apologize for the inconvenience this has caused. Please do not hesitate to contact us if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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