Product nameAnti-BPTF / FALZ antibody
See all BPTF / FALZ primary antibodies
DescriptionRabbit polyclonal to BPTF / FALZ
Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Orangutan
Synthetic peptide from a region between residue 1350 and 1400 of human BPTF/ FALZ, NP_872579.2
- Whole cell lysate from Hela cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/2000 - 1/10000. Detects a band of approximately 324 kDa (predicted molecular weight: 324 kDa).
Several other bands.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionHistone-binding component of NURF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. May also regulate transcription through direct binding to DNA or transcription factors.
Tissue specificityUbiquitously expressed, with highest levels in testis. Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex (at protein level).
Sequence similaritiesBelongs to the PBTF family.
Contains 1 bromo domain.
Contains 1 DDT domain.
Contains 2 PHD-type zinc fingers.
Developmental stageAbundantly expressed in the fetal brain. Present throughout the gray and white matter of the developing spinal cord at 18-22 gestational weeks. Expressed at low levels in adult brain and spinal cord and reexpressed in neurodegenerative diseases (at protein level).
DomainThe second PHD-type zinc finger mediates binding to histone H3K4Me3. Has specificity for trimethyllysine; introducing a mutation in the Tyr-2876 residue can induce binding to dimethyllysine.
modificationsPhosphorylation enhances DNA-binding.
Highly susceptible to proteolysis.
Cellular localizationCytoplasm. Nucleus. In brains of Alzheimer disease patients, present in a subset of amyloid-containing plaques.
- Information by UniProt
- BPTF antibody
- BPTF_HUMAN antibody
- Bromodomain and PHD finger containing transcription factor antibody
All lanes : Anti-BPTF / FALZ antibody (ab72036) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells. at 50 µg
Lane 2 : Whole cell lysate from Hela cells. at 15 µg
Lane 3 : Whole cell lysate from Hela cells. at 5 µg
Predicted band size: 324 kDa
Observed band size: 324 kDa
Various other bands are detected from 117 to 460 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BPTF / FALZ with ab72036 at 1/200 (1µg/ml). Detection: DAB.
Immunoprecipitation/ Western Blot of FALZ / BPTF.
Lane 1: ab72036 at 3µg/mg whole cell lysate for IP, and 0.1 µg/ml for subsequent WB.
Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection: Chemiluminescence with an exposure time of 1 second.
ICC/IF image of ab72036 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72036, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Zhao X et al. BPTF promotes hepatocellular carcinoma growth by modulating hTERT signaling and cancer stem cell traits. Redox Biol 20:427-441 (2019). Read more (PubMed: 30419422) »
- Pan Y et al. Bromodomain PHD-finger transcription factor promotes glioma progression and indicates poor prognosis. Oncol Rep 41:246-256 (2019). Read more (PubMed: 30542695) »