Key features and details
- Rabbit polyclonal to BPTF / FALZ
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BPTF / FALZ antibody
See all BPTF / FALZ primary antibodies
DescriptionRabbit polyclonal to BPTF / FALZ
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Orangutan
Synthetic peptide from a region between residue 1350 and 1400 of human BPTF/ FALZ, NP_872579.2
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 324 kDa (predicted molecular weight: 324 kDa).
Several other bands.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionHistone-binding component of NURF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. May also regulate transcription through direct binding to DNA or transcription factors.
Tissue specificityUbiquitously expressed, with highest levels in testis. Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex (at protein level).
Sequence similaritiesBelongs to the PBTF family.
Contains 1 bromo domain.
Contains 1 DDT domain.
Contains 2 PHD-type zinc fingers.
Developmental stageAbundantly expressed in the fetal brain. Present throughout the gray and white matter of the developing spinal cord at 18-22 gestational weeks. Expressed at low levels in adult brain and spinal cord and reexpressed in neurodegenerative diseases (at protein level).
DomainThe second PHD-type zinc finger mediates binding to histone H3K4Me3. Has specificity for trimethyllysine; introducing a mutation in the Tyr-2876 residue can induce binding to dimethyllysine.
modificationsPhosphorylation enhances DNA-binding.
Highly susceptible to proteolysis.
Cellular localizationCytoplasm. Nucleus. In brains of Alzheimer disease patients, present in a subset of amyloid-containing plaques.
- Information by UniProt
- BPTF antibody
- BPTF_HUMAN antibody
- Bromodomain and PHD finger containing transcription factor antibody
All lanes : Anti-BPTF / FALZ antibody (ab72036) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells. at 50 µg
Lane 2 : Whole cell lysate from Hela cells. at 15 µg
Lane 3 : Whole cell lysate from Hela cells. at 5 µg
Predicted band size: 324 kDa
Observed band size: 324 kDa
Various other bands are detected from 117 to 460 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BPTF / FALZ with ab72036 at 1/200 (1µg/ml). Detection: DAB.
Immunoprecipitation/ Western Blot of FALZ / BPTF.
Lane 1: ab72036 at 3µg/mg whole cell lysate for IP, and 0.1 µg/ml for subsequent WB.
Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection: Chemiluminescence with an exposure time of 1 second.
ab72036 has been referenced in 4 publications.
- Zhao X et al. BPTF promotes hepatocellular carcinoma growth by modulating hTERT signaling and cancer stem cell traits. Redox Biol 20:427-441 (2019). PubMed: 30419422
- Pan Y et al. Bromodomain PHD-finger transcription factor promotes glioma progression and indicates poor prognosis. Oncol Rep 41:246-256 (2019). PubMed: 30542695
- Richart L et al. BPTF is required for c-MYC transcriptional activity and in vivo tumorigenesis. Nat Commun 7:10153 (2016). WB ; Human . PubMed: 26729287
- Mulder KW et al. Diverse epigenetic strategies interact to control epidermal differentiation. Nat Cell Biol 14:753-63 (2012). ChIP . PubMed: 22729083