Overview

  • Product name

    Anti-Brachyury / Bry antibody [1H9A2]
    See all Brachyury / Bry primary antibodies
  • Description

    Mouse monoclonal [1H9A2] to Brachyury / Bry
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Brachyury/ Bry aa 257-309. Purified recombinant fragment, corresponding to amino acids 257-309 of Human Brachyury/ Bry, expressed in E. Coli.
    Sequence:

    PANPHPQFGGALSLPSTHSCDRYPTLRSHRSSPYPSPYAHRNNSPTYSDN SPA

  • Positive control

    • Recombinant Human Brachyury /Bry protein; HEK293 cells transfected with recombinant Human Brachyury / Bry fragment (aa 257-309); HeLa cells; Jurkat and Raji cell lysates; Human cervical cancer and rectum cancer tissues.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR136145-8 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab140661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 47 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ELISA Use at an assay dependent concentration.

Target

  • Function

    Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.
  • Involvement in disease

    Genetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis.
    T is involved in susceptibility to the development of chordoma (CHDM) [MIM:215400]. Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Note=Susceptibility to development of chordomas is due to a T gene duplication.
  • Sequence similarities

    Contains 1 T-box DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRAC_HUMAN antibody
    • Brachyury homolog antibody
    • Brachyury protein antibody
    • Bry antibody
    • MGC104817 antibody
    • Protein T antibody
    • SAVA antibody
    • T antibody
    • T brachyury homolog antibody
    • T brachyury transcription factor antibody
    • T Protein antibody
    • T, brachyury homolog (mouse) antibody
    • TFT antibody
    • Transcription factor T antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Brachyury / Bry with ab140661 at 1/200 dilution followed by DAB staining.
  • All lanes : Anti-Brachyury / Bry antibody [1H9A2] (ab140661) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : HEK293 cell lysate, transfected with recombinant Human Brachyury / Bry fragment (aa 257-309)

    Predicted band size: 47 kDa

  • Flow cytometric analysis of HeLa cells labeling Brachyury / Bry with ab140661 at 1/200 dilution (green); negative control (red).
  • Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling Brachyury / Bry with ab140661 at 1/200 dilution followed by DAB staining.
  • Anti-Brachyury / Bry antibody [1H9A2] (ab140661) at 1/500 dilution + Recombinant Human Brachyury / Bry protein

    Predicted band size: 47 kDa



    Expected MW is 31.2 kDa.
  • ELISA analysis using ab140661. Antigen = Purified recombinant fragment, corresponding to amino acids 257-309 of Human Brachyury / Bry, expressed in E. Coli.

References

This product has been referenced in:

  • Liu Q  et al. Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid. Sci Rep 8:12960 (2018). Read more (PubMed: 30154523) »
  • Jezkova J  et al. Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer. Oncotarget N/A:N/A (2016). WB, IHC . Read more (PubMed: 26862851) »
See all 2 Publications for this product

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