Anti-Brachyury / Bry antibody [6C12] (ab135254)
![Western blot - Anti-Brachyury / Bry antibody [6C12] (ab135254)](https://www.abcam.com/ps/datasheet/images/135/ab135254/Brachyury-Bry-Primary-antibodies-ab135254-6.jpg "Western blot - Anti-Brachyury / Bry antibody [6C12] (ab135254)")
Key features and details
- Mouse monoclonal [6C12] to Brachyury / Bry
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Brachyury / Bry antibody [6C12]
See all Brachyury / Bry primary antibodies -
Description
Mouse monoclonal [6C12] to Brachyury / Bry -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified recombinant fragment, corresponding to amino acids 218-352 of Human Brachyury/ Bry expressed in E.coli.
-
Positive control
- Human Brachyury / Bry recombinant protein; Brachyury / Bry transfected HEK293 cell lysate; A549 cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Protein stabilizer at 0.5%. -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6C12 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab135254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 47 kDa. | |
| Flow Cyt | 1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
-
Function
Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. -
Involvement in disease
Genetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis.
T is involved in susceptibility to the development of chordoma (CHDM) [MIM:215400]. Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Note=Susceptibility to development of chordomas is due to a T gene duplication. -
Sequence similarities
Contains 1 T-box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 6862 Human
- Omim: 601397 Human
- SwissProt: O15178 Human
- Unigene: 389457 Human
-
Alternative names
- BRAC_HUMAN antibody
- Brachyury homolog antibody
- Brachyury protein antibody
see all
Images
-
Western blot - Anti-Brachyury / Bry antibody [6C12] (ab135254)Anti-Brachyury / Bry antibody [6C12] (ab135254) at 1/500 dilution + Human Brachyury / Bry recombinant protein.
Predicted band size: 47 kDa
Expected MW is 40.3 kDa. -
![Western blot - Anti-Brachyury / Bry antibody [6C12] (ab135254)](https://www.abcam.com/ps/datasheet/images/135/ab135254/Brachyury-Bry-Primary-antibodies-ab135254-7.jpg)
Western blot - Anti-Brachyury / Bry antibody [6C12] (ab135254)All lanes : Anti-Brachyury / Bry antibody [6C12] (ab135254) at 1/500 dilution
Lane 1 : HEK293 cell lysate, non-transfected
Lane 2 : HEK293 cell lysate, transfected with Brachyury / Bry (amino acids 218-352)-hIgGFc.
Predicted band size: 47 kDa -
![Flow Cytometry - Anti-Brachyury / Bry antibody [6C12] (ab135254)](https://www.abcam.com/ps/datasheet/images/135/ab135254/Brachyury-Bry-Primary-antibodies-ab135254-4.JPG)
Flow Cytometry - Anti-Brachyury / Bry antibody [6C12] (ab135254)Flow cytometric analysis of A549 cells labelling Brachyury-Bry with ab135254 at 1/200 dilution (green) compared with a negative control (red).
Datasheets and documents
References (0)
ab135254 has not yet been referenced specifically in any publications.
