Key features and details
- Mouse monoclonal [6C12] to Brachyury / Bry
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Brachyury / Bry antibody [6C12]
See all Brachyury / Bry primary antibodies
DescriptionMouse monoclonal [6C12] to Brachyury / Bry
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Purified recombinant fragment, corresponding to amino acids 218-352 of Human Brachyury/ Bry expressed in E.coli.
- Human Brachyury / Bry recombinant protein; Brachyury / Bry transfected HEK293 cell lysate; A549 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Protein stabilizer at 0.5%.
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab135254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 47 kDa.|
|Flow Cyt||1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionInvolved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.
Involvement in diseaseGenetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis.
T is involved in susceptibility to the development of chordoma (CHDM) [MIM:215400]. Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Note=Susceptibility to development of chordomas is due to a T gene duplication.
Sequence similaritiesContains 1 T-box DNA-binding domain.
- Information by UniProt
- BRAC_HUMAN antibody
- Brachyury homolog antibody
- Brachyury protein antibody
Anti-Brachyury / Bry antibody [6C12] (ab135254) at 1/500 dilution + Human Brachyury / Bry recombinant protein.
Predicted band size: 47 kDa
Expected MW is 40.3 kDa.
All lanes : Anti-Brachyury / Bry antibody [6C12] (ab135254) at 1/500 dilution
Lane 1 : HEK293 cell lysate, non-transfected
Lane 2 : HEK293 cell lysate, transfected with Brachyury / Bry (amino acids 218-352)-hIgGFc.
Predicted band size: 47 kDa
Flow cytometric analysis of A549 cells labelling Brachyury-Bry with ab135254 at 1/200 dilution (green) compared with a negative control (red).
ab135254 has not yet been referenced specifically in any publications.